ArticleAntimicrobial agents and chemotherapy2013
Efficacy of AiiM, an N-acylhomoserine lactonase, against Pseudomonas aeruginosa in a mouse model of acute pneumonia.
Article in Antimicrobial agents and chemotherapy, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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22 citing papers in PubMed, 43 citations in OpenAlex.
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- Engineering quorum quenching acylases with improved kinetic and biochemical properties.Protein science : a publication of the Protein Society · 2024Article
- N-acyl homoserine lactonase attenuates the virulence ofAnimal nutrition (Zhongguo xu mu shou yi xue hui) · 2023Article
- Supplemental N-acyl homoserine lactonase alleviates intestinal disruption and improves gut microbiota in broilers challenged by Salmonella Typhimurium.Journal of animal science and biotechnology · 2023Article
- The Association between Biofilm Formation and Antimicrobial Resistance with Possible Ingenious Bio-Remedial Approaches.Antibiotics (Basel, Switzerland) · 2022Review
- Pseudomonas aeruginosa Quorum Sensing.Advances in experimental medicine and biology · 2022Article
- Article
- A Quorum-Sensing Inhibitor Strain ofMarine drugs · 2019Article
- Quorum quenching activity ofInfection and drug resistance · 2019Article
- Recent Advances in Anti-virulence Therapeutic Strategies With a Focus on Dismantling Bacterial Membrane Microdomains, Toxin Neutralization, Quorum-Sensing Interference and Biofilm Inhibition.Frontiers in cellular and infection microbiology · 2019Review
- Small Molecule Anti-biofilm Agents Developed on the Basis of Mechanistic Understanding of Biofilm Formation.Frontiers in chemistry · 2019Review
- AiiM Lactonase Strongly Reduces Quorum Sensing Controlled Virulence Factors in Clinical Strains ofFrontiers in microbiology · 2019Article
- Characterization of a novel N-acylhomoserine lactonase, AidP, from Antarctic Planococcus sp.Microbial cell factories · 2018Article
- PvdQ Quorum Quenching Acylase AttenuatesFrontiers in cellular and infection microbiology · 2018Article
- Pitfalls associated with evaluating enzymatic quorum quenching activity: the case of MomL and its effect onPeerJ · 2017Article
- Aii810, a Novel Cold-AdaptedFrontiers in microbiology · 2017Article
- Fast, Continuous, and High-Throughput (Bio)Chemical Activity Assay for N-Acyl-l-Homoserine Lactone Quorum-Quenching Enzymes.Applied and environmental microbiology · 2016Article
- Interfering with Bacterial Quorum Sensing.Perspectives in medicinal chemistry · 2016Article
- Rhodococcus erythropolis BG43 Genes Mediating Pseudomonas aeruginosa Quinolone Signal Degradation and Virulence Factor Attenuation.Applied and environmental microbiology · 2015Article
Corrections and comments
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Authors and funding
12 authors at 4 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Quorum sensing (QS) in Pseudomonas aeruginosa regulates the production of many virulence factors and plays an important role in the pathogenesis of P. aeruginosa infection. N-acyl homoserine lactones (AHL) are major QS signal molecules. Recently, a novel AHL-lactonase enzyme, AiiM, has been identified. The aim of this study was to evaluate the effect of AiiM on the virulence of P. aeruginosa in a mouse model of acute pneumonia. We developed a P. aeruginosa PAO1 strain harboring an AiiM-expressing plasmid. The production of several virulence factors by the AiiM-expressing strain was examined. Mice were intratracheally infected with an AiiM-expressing PAO1 strain. Lung histopathology, bacterial burden, and bronchoalveolar lavage (BAL) fluid were assessed at 24 h postinfection. AiiM expression in PAO1 reduced production of AHL-mediated virulence factors and attenuated cytotoxicity against human lung epithelial cells. In a mouse model of acute pneumonia, AiiM expression reduced lung injury and greatly improved the survival rates. The levels of proinflammatory cytokines and myeloperoxidase activity in BAL fluid were significantly lower in mice infected with AiiM-expressing PAO1. Thus, AiiM can strongly attenuate P. aeruginosa virulence in a mammalian model and is a potential candidate for use as a therapeutic agent against P. aeruginosa infection.
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