ArticleThe Journal of biological chemistry2013
Simultaneous surface display and secretion of proteins from mammalian cells facilitate efficient in vitro selection and maturation of antibodies.
Article in The Journal of biological chemistry, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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Who cites it
23 citing papers in PubMed.
- Optimizing co-expression strategies for the simultaneous display and secretion of monoclonal antibodies in CHO cells.Scientific reports · 2025Article
- A Targeted Integration-Based CHO Cell Platform for Simultaneous Antibody Display and Secretion.Antibodies (Basel, Switzerland) · 2025Article
- Dostarlimab in the treatment of mismatch repair deficient recurrent or advanced endometrial cancer.Cancer pathogenesis and therapy · 2024Review
- Construction and application of a multifunctional CHO cell platform utilizing Cre/Frontiers in bioengineering and biotechnology · 2023Article
- Clinical landscape of LAG-3-targeted therapy.Immuno-oncology technology · 2022Review
- Affinity maturation: highlights in the application of in vitro strategies for the directed evolution of antibodies.Emerging topics in life sciences · 2021Article
- Review
- Amber suppression coupled with inducible surface display identifies cells with high recombinant protein productivity.Biotechnology and bioengineering · 2019Article
- Enhancers Improve the AID-Induced Hypermutation in Episomal Vector for Antibody Affinity Maturation in Mammalian Cell Display.Antibodies (Basel, Switzerland) · 2018Article
- Both Intrinsically Disordered Regions and Structural Domains Evolve Rapidly in Immune-Related Mammalian Proteins.International journal of molecular sciences · 2018Article
- Transient AID expression for in situ mutagenesis with improved cellular fitness.Scientific reports · 2018Article
- Identification of high affinity HER2 binding antibodies using CHO Fab surface display.Protein engineering, design & selection : PEDS · 2018Article
- Inefficient Ribosomal Skipping Enables Simultaneous Secretion and Display of Proteins in Saccharomyces cerevisiae.ACS synthetic biology · 2017Article
- Article
- Immunogenomic engineering of a plug-and-(dis)play hybridoma platform.Nature communications · 2016Article
- A Lentiviral Vector Allowing Physiologically Regulated Membrane-anchored and Secreted Antibody Expression Depending on B-cell Maturation Status.Molecular therapy : the journal of the American Society of Gene Therapy · 2015Article
- A switchable yeast display/secretion system.Protein engineering, design & selection : PEDS · 2015Article
- Nucleotide insertions and deletions complement point mutations to massively expand the diversity created by somatic hypermutation of antibodies.The Journal of biological chemistry · 2014Article
- Advances in the directed evolution of proteins.Current opinion in chemical biology · 2014Review
- A dual-mode surface display system for the maturation and production of monoclonal antibodies in glyco-engineered Pichia pastoris.PloS one · 2013Article
Corrections and comments
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Authors and funding
9 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
A mammalian expression system has been developed that permits simultaneous cell surface display and secretion of the same protein through alternate splicing of pre-mRNA. This enables a flexible system for in vitro protein evolution in mammalian cells where the displayed protein phenotype remains linked to genotype, but with the advantage of soluble protein also being produced without the requirement for any further recloning to allow a wide range of assays, including biophysical and cell-based functional assays, to be used during the selection process. This system has been used for the simultaneous surface presentation and secretion of IgG during antibody discovery and maturation. Presentation and secretion of monomeric Fab can also be achieved to minimize avidity effects. Manipulation of the splice donor site sequence enables control of the relative amounts of cell surface and secreted antibody. Multi-domain proteins may be presented and secreted in different formats to enable flexibility in experimental design, and secreted proteins may be produced with epitope tags to facilitate high-throughput testing. This system is particularly useful in the context of in situ mutagenesis, as in the case of in vitro somatic hypermutation.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.