ArticleMolecular and cellular biology2013
Rpd3- and spt16-mediated nucleosome assembly and transcriptional regulation on yeast ribosomal DNA genes.
Article in Molecular and cellular biology, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed, 27 citations in OpenAlex.
- Sir proteins impede, but do not prevent, access to silent chromatin in living Saccharomyces cerevisiae.Scientific reports · 2026Article
- Psoralen Crosslinking-Chromatin Endogenous Cleavage Assay to Examine Histone DNA Interactions of Active and Inactive rRNA Genes.Methods in molecular biology (Clifton, N.J.) · 2025Article
- Establishment of closed 35S ribosomal RNA gene chromatin in stationary Saccharomyces cerevisiae cells.Nucleic acids research · 2024Article
- Acidic growth conditions stabilize the ribosomal RNA gene cluster and extend lifespan through noncoding transcription repression.Genes to cells : devoted to molecular & cellular mechanisms · 2024Article
- Single-molecule study reveals Hmo1, not Hho1, promotes chromatin assembly in budding yeast.mBio · 2023Article
- Targeting of Hmo1 to subcompartments of the budding yeast nucleolus.Molecular biology of the cell · 2023Article
- Establishment and Maintenance of Open Ribosomal RNA Gene Chromatin States in Eukaryotes.Methods in molecular biology (Clifton, N.J.) · 2022Review
- Coordinated Control of rRNA Processing by RNA Polymerase I.Trends in genetics : TIG · 2019Review
- Review
- Nucleolus and chromatin.Histochemistry and cell biology · 2018Review
- Structure and function of the histone chaperone FACT - Resolving FACTual issues.Biochimica et biophysica acta. Gene regulatory mechanisms · 2018Review
- Feedback regulation of ribosome assembly.Current genetics · 2018Review
- Transcription factors that influence RNA polymerases I and II: To what extent is mechanism of action conserved?Biochimica et biophysica acta. Gene regulatory mechanisms · 2017Review
- Morphology of nuclear transcription.Histochemistry and cell biology · 2016Review
- Spt6 Is Essential for rRNA Synthesis by RNA Polymerase I.Molecular and cellular biology · 2015Article
- Review
- Article
- Regulation of chromatin structure and function: insights into the histone chaperone FACT.Cell cycle (Georgetown, Tex.)Review
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Authors and funding
7 authors at 1 institution in 1 country.
Funding
Abstract
Ribosomal DNA (rDNA) genes in eukaryotes are organized into multicopy tandem arrays and transcribed by RNA polymerase I. During cell proliferation, ∼50% of these genes are active and have a relatively open chromatin structure characterized by elevated accessibility to psoralen cross-linking. In Saccharomyces cerevisiae, transcription of rDNA genes becomes repressed and chromatin structure closes when cells enter the diauxic shift and growth dramatically slows. In this study, we found that nucleosomes are massively depleted from the active rDNA genes during log phase and reassembled during the diauxic shift, largely accounting for the differences in psoralen accessibility between active and inactive genes. The Rpd3L histone deacetylase complex was required for diauxic shift-induced H4 and H2B deposition onto rDNA genes, suggesting involvement in assembly or stabilization of the entire nucleosome. The Spt16 subunit of FACT, however, was specifically required for H2B deposition, suggesting specificity for the H2A/H2B dimer. Miller chromatin spreads were used for electron microscopic visualization of rDNA genes in an spt16 mutant, which was found to be deficient in the assembly of normal nucleosomes on inactive genes and the disruption of nucleosomes on active genes, consistent with an inability to fully reactivate polymerase I (Pol I) transcription when cells exit stationary phase.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.