ArticleAnalytical chemistry2013
Glycomic analysis using glycoprotein immobilization for glycan extraction.
Article in Analytical chemistry, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 33 papers.
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Who cites it
33 citing papers in PubMed, 71 citations in OpenAlex.
- Mass Spectrometry-Based Comparative Analysis ofACS chemical neuroscience · 2025Article
- Deciphering disease-specific glycosylation: unraveling diabetes subtypes through serum glycopattern.Analytical and bioanalytical chemistry · 2025Article
- SIAE-Mediated Loss of Sialic Acid Acetylation Contributes to Ulcerative Colitis.Journal of inflammation research · 2025Article
- Mass spectrometry analysis of intact proteinJournal of Zhejiang University. Science. B · 2024Article
- Quantitative analysis of fucosylated glycoproteins by immobilized lectin-affinity fluorescent labeling.RSC advances · 2023Article
- Aberrant Fucosylation of Saliva Glycoprotein Defining Lung Adenocarcinomas Malignancy.ACS omega · 2022Article
- A biotechnological tool for glycoprotein desialylation based on immobilized neuraminidase fromBiochemistry and biophysics reports · 2021Article
- Impact of Expressing Cells on Glycosylation and Glycan of the SARS-CoV-2 Spike Glycoprotein.ACS omega · 2021Article
- NIST Interlaboratory Study on Glycosylation Analysis of Monoclonal Antibodies: Comparison of Results from Diverse Analytical Methods.Molecular & cellular proteomics : MCP · 2020Article
- Deciphering the Roles of N-Glycans on Collagen-Platelet Interactions.Journal of proteome research · 2019Article
- The ammonia-catalyzed release of glycoprotein N-glycans.Glycoconjugate journal · 2018Article
- Identification of Sialic Acid Linkages on Intact Glycopeptides via Differential Chemical Modification Using IntactGIG-HILIC.Journal of the American Society for Mass Spectrometry · 2018Article
- Site-Specific Profiling of Serum Glycoproteins Using N-Linked Glycan and Glycosite Analysis Revealing Atypical N-Glycosylation Sites on Albumin and α-1B-Glycoprotein.Analytical chemistry · 2018Article
- N-glycan in cockroach allergen regulates human basophil function.Immunity, inflammation and disease · 2017Article
- High-throughput analysis of N-glycans using AutoTip via glycoprotein immobilization.Scientific reports · 2017Article
- Modification of Sialic Acids on Solid Phase: Accurate Characterization of Protein Sialylation.Analytical chemistry · 2017Article
- Simultaneous quantification of N- and O-glycans using a solid-phase method.Nature protocols · 2017Article
- Recent Advances in the Analysis of Complex Glycoproteins.Analytical chemistry · 2017Review
- Discovery of Non-invasive Glycan Biomarkers for Detection and Surveillance of Gastric Cancer.Journal of Cancer · 2017Article
- Simultaneous analyses of N-linked and O-linked glycans of ovarian cancer cells using solid-phase chemoenzymatic method.Clinical proteomics · 2017Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
Glycosylation is one of the most common protein modifications and is involved in many functions of glycoproteins. Investigating aberrant protein glycosylation associated with diseases is useful in improving disease diagnostics. Due to the nontemplate nature of glycan biosynthesis, the glycans attached to glycoproteins are enormously complex; thus, a method for comprehensive analysis of glycans from biological or clinical samples is needed. Here, we describe a novel method for glycomic analysis using glycoprotein immobilization for glycan extraction (GIG). Proteins or peptides from complex samples were first immobilized on solid support, and other nonconjugated molecules were removed. Glycans were enzymatically or chemically modified on solid phase before releasing from glycoproteins/glycopeptides for mass spectrometry analysis. The method was applied to the glycomic analysis of both N- and O-glycans.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.