ArticleReproductive biology and endocrinology : RB&E2013
Proteomic analysis of human spermatozoa proteins with oxidative stress.
Article in Reproductive biology and endocrinology : RB&E, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 48 papers, 1 of them a synthesis that pooled it.
What it found
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Who cites it
48 citing papers in PubMed, 1 synthesis or guideline pooled it, 131 citations in OpenAlex.
- Exploring Human Sperm Metabolism and Male Infertility: A Systematic Review of Genomics, Proteomics, Metabolomics, and Imaging Techniques.International journal of molecular sciences · 2025Pooled it
- Epidemiology, Etiopathogenesis, Diagnosis, and Treatment of Male Infertility-Current Trends and Future Directions: A Narrative Review.Medicina (Kaunas, Lithuania) · 2026Review
- Metabolic pathways fueling human spermatozoa capacitation and hyperactivation: a roadmap for spermatozoa bioenergetics.Frontiers in endocrinology · 2026Review
- Experimental Evidence That Prenatal and Postnatal Developmental Stress Affects the Adult Seminal Fluid Proteome in a Precocial Bird.Molecular ecology · 2026Article
- Spermatozoa Proteins Involved in ROS Generation and Antioxidant Defense Are Differentially Acetylated in Idiopathic Infertility.Antioxidants (Basel, Switzerland) · 2025Article
- Impact of Oxidative Stress and Antioxidants on Semen Quality in Dogs.Animals : an open access journal from MDPI · 2025Review
- Beneficial Effects of Resveratrol on Testicular Functions: Focus on Its Antioxidant Properties.Cells · 2025Review
- Age-Associated Proteomic Changes in Human Spermatozoa.International journal of molecular sciences · 2025Article
- The impact, mechanisms and prevention strategies of environmental endocrine disruptors on male reproductive health.Frontiers in endocrinology · 2025Review
- Oxidative Stress-Associated Male Infertility: Current Diagnostic and Therapeutic Approaches.Medicina (Kaunas, Lithuania) · 2024Review
- Molecular Biomarkers of Canine Reproductive Functions.Current issues in molecular biology · 2024Review
- Non-Invasive Diagnostics of Male Spermatogenesis from Seminal Plasma: Seminal Proteins.Diagnostics (Basel, Switzerland) · 2023Review
- The Ubiquitin-Proteasome System Participates in Sperm Surface Subproteome Remodeling during Boar Sperm Capacitation.Biomolecules · 2023Article
- Unraveling the harmful effect of oxidative stress on male fertility: A mechanistic insight.Frontiers in endocrinology · 2023Review
- TMT-Based Proteomic Analysis of Human Spermatozoa from Unexplained Recurrent Miscarriage Patients before and after Oral Antioxidant Treatment.Biomedicines · 2022Article
- Proteomic Analysis of Intracellular and Membrane-Associated Fractions of Canine (Animals : an open access journal from MDPI · 2022Article
- Molecular Interactions Associated with Oxidative Stress-Mediated Male Infertility: Sperm and Seminal Plasma Proteomics.Advances in experimental medicine and biology · 2022Review
- A novel posttranslational modification of histone, H3 S-sulfhydration, is down-regulated in asthenozoospermic sperm.Journal of assisted reproduction and genetics · 2021Article
- Mitochondrial Functionality in Male Fertility: From Spermatogenesis to Fertilization.Antioxidants (Basel, Switzerland) · 2021Review
- Sperm Functional Genome Associated With Bull Fertility.Frontiers in veterinary science · 2021Review
Corrections and comments
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Authors and funding
8 authors at 4 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundOxidative stress plays a key role in the etiology of male infertility. Significant alterations in the sperm proteome are associated with poor semen quality. The aim of the present study was to examine if elevated levels of reactive oxygen species cause an alteration in the proteomic profile of spermatozoa.
methodsThis prospective study consisted of 52 subjects: 32 infertile men and 20 normal donors. Seminal ejaculates were classified as ROS+ or ROS- and evaluated for their proteomic profile. Samples were pooled and subjected to LC-MS/MS analysis through in-solution digestion of proteins for peptide characterization. The expression profile of proteins present in human spermatozoa was examined using proteomic and bioinformatic analysis to elucidate the regulatory pathways of oxidative stress.
resultsOf the 74 proteins identified, 10 proteins with a 2-fold difference were overexpressed and 5 were underexpressed in the ROS+ group; energy metabolism and regulation, carbohydrate metabolic processes such as gluconeogenesis and glycolysis, protein modifications and oxidative stress regulation were some of the metabolic processes affected in ROS+ group.
conclusionsWe have identified proteins involved in a variety of functions associated with response and management of oxidative stress. In the present study we focused on proteins that showed a high degree of differential expression and thus, have a greater impact on the fertilizing potential of the spermatozoa. While proteomic analyses identified the potential biomarkers, further studies through Western Blot are necessary to validate the biomarker status of the proteins in pathological conditions.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.