ArticleJournal of cell science2013
Vinculin tension distributions of individual stress fibers within cell-matrix adhesions.
Article in Journal of cell science, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 33 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
33 citing papers in PubMed, 64 citations in OpenAlex.
- Guidance for 3D traction force microscopy today and in the next decade.Nature methods · 2026Review
- Linking molecular tension and cellular tractions: a multiscale approach to focal adhesion mechanics.Communications biology · 2026Article
- VCL/ICAM-1 pathway is associated with lung inflammatory damage in SARS-CoV-2 Omicron infection.Nature communications · 2025Article
- Evaluation of focal adhesion mediated subcellular curvature sensing in response to engineered extracellular matrix.Biointerphases · 2023Article
- FilamentSensor 2.0: An open-source modular toolbox for 2D/3D cytoskeletal filament tracking.PloS one · 2023Article
- The biochemical composition of the actomyosin network sets the magnitude of cellular traction forces.Molecular biology of the cell · 2021Article
- Articular Chondrocyte Phenotype Regulation through the Cytoskeleton and the Signaling Processes That Originate from or Converge on the Cytoskeleton: Towards a Novel Understanding of the Intersection between Actin Dynamics and Chondrogenic Function.International journal of molecular sciences · 2021Review
- Runx3 Induces a Cell Shape Change and Suppresses Migration and Metastasis of Melanoma Cells by Altering a Transcriptional Profile.International journal of molecular sciences · 2021Article
- Cofilin is required for polarization of tension in stress fiber networks during migration.Journal of cell science · 2020Article
- Curvotaxis directs cell migration through cell-scale curvature landscapes.Nature communications · 2018Article
- Actomyosin stress fiber subtypes have unique viscoelastic properties and roles in tension generation.Molecular biology of the cell · 2018Article
- Live cell imaging reveals focal adhesions mechanoresponses in mammary epithelial cells under sustained equibiaxial stress.Scientific reports · 2018Article
- Activation of ROCK and MLCK tunes regional stress fiber formation and mechanics via preferential myosin light chain phosphorylation.Molecular biology of the cell · 2017Article
- Multiplexing molecular tension sensors reveals piconewton force gradient across talin-1.Nature methods · 2017Article
- Dissipation of contractile forces: the missing piece in cell mechanics.Molecular biology of the cell · 2017Article
- Geometry and network connectivity govern the mechanics of stress fibers.Proceedings of the National Academy of Sciences of the United States of America · 2017Article
- Coordination between Intra- and Extracellular Forces Regulates Focal Adhesion Dynamics.Nano letters · 2017Article
- Bio-Nano-Magnetic Materials for Localized Mechanochemical Stimulation of Cell Growth and Death.Advanced materials (Deerfield Beach, Fla.) · 2016Article
- Relationship between cell stiffness and stress fiber amount, assessed by simultaneous atomic force microscopy and live-cell fluorescence imaging.Biomechanics and modeling in mechanobiology · 2016Article
- Mechanosensitive components of integrin adhesions: Role of vinculin.Experimental cell research · 2016Review
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
Actomyosin stress fibers (SFs) enable cells to exert traction on planar extracellular matrices (ECMs) by tensing focal adhesions (FAs) at the cell-ECM interface. Although it is widely appreciated that the spatial and temporal distribution of these tensile forces play key roles in polarity, motility, fate choice, and other defining cell behaviors, virtually nothing is known about how an individual SF quantitatively contributes to tensile loads borne by specific molecules within associated FAs. We address this key open question by using femtosecond laser ablation to sever single SFs in cells while tracking tension across vinculin using a molecular optical sensor. We show that disruption of a single SF reduces tension across vinculin in FAs located throughout the cell, with enriched vinculin tension reduction in FAs oriented parallel to the targeted SF. Remarkably, however, some subpopulations of FAs exhibit enhanced vinculin tension upon SF irradiation and undergo dramatic, unexpected transitions between tension-enhanced and tension-reduced states. These changes depend strongly on the location of the severed SF, consistent with our earlier finding that different SF pools are regulated by distinct myosin activators. We critically discuss the extent to which these measurements can be interpreted in terms of whole-FA tension and traction and propose a model that relates SF tension to adhesive loads and cell shape stability. These studies represent the most direct and high-resolution intracellular measurements of SF contributions to tension on specific FA proteins to date and offer a new paradigm for investigating regulation of adhesive complexes by cytoskeletal force.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.