ArticleMolecular reproduction and development2013
Retention of exogenous mRNAs selectively in the germ cells of the sea urchin requires only a 5'-cap and a 3'-UTR.
Article in Molecular reproduction and development, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
9 citing papers in PubMed, 13 citations in OpenAlex.
- A conserved node in the regulation of Vasa between an induced and an inherited program of primordial germ cell specification.Developmental biology · 2022Article
- A single cell RNA sequencing resource for early sea urchin development.Development (Cambridge, England) · 2020Article
- Methods to label, isolate, and image sea urchin small micromeres, the primordial germ cells (PGCs).Methods in cell biology · 2019Review
- Differential Nanos 2 protein stability results in selective germ cell accumulation in the sea urchin.Developmental biology · 2016Article
- Germ Line Versus Soma in the Transition from Egg to Embryo.Current topics in developmental biology · 2015Review
- The biology of the germ line in echinoderms.Molecular reproduction and development · 2014Review
- Deadenylase depletion protects inherited mRNAs in primordial germ cells.Development (Cambridge, England) · 2014Article
- Migration of sea urchin primordial germ cells.Developmental dynamics : an official publication of the American Association of Anatomists · 2014Article
- Selective accumulation of germ-line associated gene products in early development of the sea star and distinct differences from germ-line development in the sea urchin.Developmental dynamics : an official publication of the American Association of Anatomists · 2014Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
The abundance of an mRNA in a cell depends on its overall rates of synthesis and decay. RNA stability is an important element in the regulation of gene expression, and is achieved by a variety of processes including specific recruitment of nucleases and RNAi-associated mechanisms. These mechanisms are particularly important in stem cells, which, in many cases, have attenuated transcription. Here we report that exogenous mRNA injected into fertilized eggs of the sea urchin is selectively retained in the small micromeres, which contribute to the germ line in this organism, beginning in blastulae, when compared to adjacent somatic cells. We show that modification of this exogenous RNA using cap analogs and poly-adenosine tail deletions do not affect its selective retention in the small micromeres, but removal of the cap or of the 3'-untranslated region eliminates any selective mRNA retention in the presumptive germ line. Our results illuminate a likely ancient mechanism used by stem cells to prolong the lifespan of RNAs-either through RNA protection or by the absence of basic RNA degradation mechanisms, which are employed by most other cells of an organism.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.