ArticleJournal of clinical laboratory analysis2013
Incidence of pulmonary aspergillosis and correlation of conventional diagnostic methods with nested PCR and real-time PCR assay using BAL fluid in intensive care unit patients.
Article in Journal of clinical laboratory analysis, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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13 citing papers in PubMed, 24 citations in OpenAlex.
- Clinical characteristics and etiological analysis of deep fungal infections in a general hospital in Southwest China (2020-2024).Frontiers in public health · 2026Article
- Diagnostic and prognostic roles of interferon-λ1 and interferon-λ3 in bronchoalveolar lavage fluid and plasma in non-neutropenic patients with invasive pulmonary aspergillosis.Microbiology spectrum · 2025Article
- Hematological Indices in COVID-19 Patients with Rhinosinusitis Mucormycosis.Iranian journal of otorhinolaryngology · 2024Article
- NLRP3, NLRC4 and NLRC5 Gene Polymorphisms Associate with Susceptibility of Pulmonary Aspergillosis in Non-Neutropenic Patients.Journal of clinical medicine · 2022Article
- Invasive Pulmonary Aspergillosis DiagnosisFrontiers in medicine · 2022Article
- Deep Fungal Infections Among General Hospital Inpatients in Southwestern China: A 5-Year Retrospective Study.Frontiers in public health · 2022Article
- First report of invasiveClinical case reports · 2021Article
- Molecular typing of clinical and environmental Aspergillus fumigatus isolates from Iran using microsatellites.Current medical mycology · 2021Article
- A Microbial World: Could Metagenomic Next-Generation Sequencing Be Involved in Acute Respiratory Failure?Frontiers in cellular and infection microbiology · 2021Article
- BAL fluid analysis in the identification of infectious agents in patients with hematological malignancies and pulmonary infiltrates.Folia microbiologica · 2020Article
- Detection of Aspergillus flavus and A. fumigatus in Bronchoalveolar Lavage Specimens of Hematopoietic Stem Cell Transplants and Hematological Malignancies Patients by Real-Time Polymerase Chain Reaction, Nested PCR and Mycological Assays.Jundishapur journal of microbiology · 2015Article
- Molecular detection of Candida spp. and Aspergillus fumigatus in bronchoalveolar lavage fluid of patients with ventilator-associated pneumonia.Journal of research in medical sciences : the official journal of Isfahan University of Medical Sciences · 2014Article
- Use of mycological, nested PCR, and real-time PCR methods on BAL fluids for detection of Aspergillus fumigatus and A. flavus in solid organ transplant recipients.Mycopathologia · 2013Article
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Authors and funding
5 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundAlthough the incidence of invasive aspergillosis in the intensive care unit (ICU) is scarce, it has emerged as major problems in critically ill patients. In this study, the incidence of pulmonary aspergillosis (PA) in ICU patients has evaluated and direct microscopy and culture has compared with nested polymerase chain reaction (PCR) and real-time PCR for detection of Aspergillus fumigatus and A. flavus in bronchoalveolar lavage (BAL) samples of the patients.
methodsThirty BAL samples obtained from ICU patients during a 16-month period were subjected to direct examinations on 20% potassium hydroxide (KOH) and culture on two culture media. Nested PCR targeting internal transcribed spacer ribosomal DNA and TaqMan real-time PCR assay targeting β-tubulin gene were used for the detection of A. fumigatus and A. flavus.
resultsOf 30 patients, 60% were men and 40% were women. The diagnosis of invasive PA was probable in 1 (3%), possible in 11 (37%), and not IPA in 18 (60%). Nine samples were positive in nested PCR including seven samples by A. flavus and two by A. fumigatus specific primers. The lowest amount of DNA that TaqMan real-time PCR could detect was ≥40 copy numbers. Only one of the samples had a positive result of A. flavus real-time PCR with Ct value of 37.5.
conclusionsAlthough a significant number of specimens were positive in nested PCR, results of this study showed that establishment of a correlation between the conventional methods with nested PCR and real-time PCR needs more data confirmed by a prospective study with a larger sample group.
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