ArticlePlant physiology2013
Vacuolar transport of the medicinal alkaloids from Catharanthus roseus is mediated by a proton-driven antiport.
Article in Plant physiology, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
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Who cites it
25 citing papers in PubMed, 71 citations in OpenAlex.
- Research Progress on Preparation, Transformation and Application of Protoplasts Derived from Medicinal Plants.Plants (Basel, Switzerland) · 2026Review
- Phenotypic Variability and Anticancer Alkaloid Profiles ofPlants (Basel, Switzerland) · 2025Article
- Discovery of ElABCG39: a key player in ingenol transmembrane efflux identified through genome-wide analysis of ABC transporters in Euphorbia lathyris L.Plant cell reports · 2024Article
- Quantitative Single-Cell Mass Spectrometry Provides a Highly Resolved Analysis of Natural Product Biosynthesis Partitioning in Plants.Journal of the American Chemical Society · 2024Article
- The leaf idioblastome of the medicinal plant Catharanthus roseus is associated with stress resistance and alkaloid metabolism.Journal of experimental botany · 2024Article
- Article
- Cell type matters: competence for alkaloid metabolism differs in two seed-derived cell strains of Catharanthus roseus.Protoplasma · 2023Article
- De Novo transcriptome assembly and differential expression analysis of catharanthus roseus in response to salicylic acid.Scientific reports · 2022Article
- Characterization of Triterpene Saponin Glycyrrhizin Transport byPlants (Basel, Switzerland) · 2022Article
- Exploration of the Mechanisms of Differential Indole Alkaloid Biosynthesis in Dedifferentiated and Cambial Meristematic Cells ofFrontiers in genetics · 2022Article
- Transcriptome repository of North-Western Himalayan endangered medicinal herbs: a paramount approach illuminating molecular perspective of phytoactive molecules and secondary metabolism.Molecular genetics and genomics : MGG · 2021Review
- The GORKY glycoalkaloid transporter is indispensable for preventing tomato bitterness.Nature plants · 2021Article
- Membrane transporters: the key drivers of transport of secondary metabolites in plants.Plant cell reports · 2021Review
- The expression of Terpenoid Indole Alkaloid (TIAs) pathway genes in Catharanthus roseus in response to salicylic acid treatment.Molecular biology reports · 2020Article
- Genome Wild Analysis and Molecular Understanding of the Aquaporin Diversity in Olive Trees (International journal of molecular sciences · 2020Article
- Skin transcriptional profiles in Oophaga poison frogs.Genetics and molecular biology · 2020Article
- Membrane-Bound Class III Peroxidases: Unexpected Enzymes with Exciting Functions.International journal of molecular sciences · 2018Review
- Transcriptomics comparison reveals the diversity of ethylene and methyl-jasmonate in roles of TIA metabolism in Catharanthus roseus.BMC genomics · 2018Article
- Article
- Identification of Iridoid Glucoside Transporters in Catharanthus roseus.Plant & cell physiology · 2017Article
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Catharanthus roseus is one of the most studied medicinal plants due to the interest in their dimeric terpenoid indole alkaloids (TIAs) vinblastine and vincristine, which are used in cancer chemotherapy. These TIAs are produced in very low levels in the leaves of the plant from the monomeric precursors vindoline and catharanthine and, although TIA biosynthesis is reasonably well understood, much less is known about TIA membrane transport mechanisms. However, such knowledge is extremely important to understand TIA metabolic fluxes and to develop strategies aimed at increasing TIA production. In this study, the vacuolar transport mechanism of the main TIAs accumulated in C. roseus leaves, vindoline, catharanthine, and α-3',4'-anhydrovinblastine, was characterized using a tonoplast vesicle system. Vindoline uptake was ATP dependent, and this transport activity was strongly inhibited by NH4(+) and carbonyl cyanide m-chlorophenyl hydrazine and was insensitive to the ATP-binding cassette (ABC) transporter inhibitor vanadate. Spectrofluorimetry assays with a pH-sensitive fluorescent probe showed that vindoline and other TIAs indeed were able to dissipate an H(+) gradient preestablished across the tonoplast by either vacuolar H(+)-ATPase or vacuolar H(+)-pyrophosphatase. The initial rates of H(+) gradient dissipation followed Michaelis-Menten kinetics, suggesting the involvement of mediated transport, and this activity was species and alkaloid specific. Altogether, our results strongly support that TIAs are actively taken up by C. roseus mesophyll vacuoles through a specific H(+) antiport system and not by an ion-trap mechanism or ABC transporters.
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