ArticleNucleic acids research2013
Quantitative and simultaneous translational control of distinct mammalian mRNAs.
Article in Nucleic acids research, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed, 39 citations in OpenAlex.
- Structural insights into lab-coevolved RNA-RBP pairs and applications of synthetic riboswitches in cell-free system.Nucleic acids research · 2025Article
- An effective live-attenuated Zika vaccine candidate with a modified 5' untranslated region.NPJ vaccines · 2023Article
- Synthetic circular RNA switches and circuits that control protein expression in mammalian cells.Nucleic acids research · 2023Article
- Sensing intracellular signatures with synthetic mRNAs.RNA biology · 2023Review
- Directed evolution of orthogonal RNA-RBP pairs through library-vs-library in vitro selection.Nucleic acids research · 2022Article
- Protein-Based Systems for Translational Regulation of Synthetic mRNAs in Mammalian Cells.Life (Basel, Switzerland) · 2021Review
- Overcoming the design, build, test bottleneck for synthesis of nonrepetitive protein-RNA cassettes.Nature communications · 2021Article
- Systematic use of synthetic 5'-UTR RNA structures to tune protein translation improves yield and quality of complex proteins in mammalian cell factories.Nucleic acids research · 2020Article
- N 1-Methylpseudouridine substitution enhances the performance of synthetic mRNA switches in cells.Nucleic acids research · 2020Article
- Caliciviral protein-based artificial translational activator for mammalian gene circuits with RNA-only delivery.Nature communications · 2020Article
- Numerical operations in living cells by programmable RNA devices.Science advances · 2019Article
- Synthetic Switches and Regulatory Circuits in Plants.Plant physiology · 2019Review
- Synthetic switch to minimize CRISPR off-target effects by self-restricting Cas9 transcription and translation.Nucleic acids research · 2019Article
- Synthetic RNA-based logic computation in mammalian cells.Nature communications · 2018Article
- Synthetic switch-based baculovirus for transgene expression control and selective killing of hepatocellular carcinoma cells.Nucleic acids research · 2018Article
- Design of RNA hairpin modules that predictably tune translation in yeast.Synthetic biology (Oxford, England) · 2018Article
- Synthetic mRNA devices that detect endogenous proteins and distinguish mammalian cells.Nucleic acids research · 2017Article
- High-resolution Identification and Separation of Living Cell Types by Multiple microRNA-responsive Synthetic mRNAs.Scientific reports · 2016Article
- RNA-based gene circuits for cell regulation.Proceedings of the Japan Academy. Series B, Physical and biological sciences · 2016Review
- Expanding the synthetic ribonucleoprotein world in cells.Nature methods · 2014Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The introduction of multiple genes into cells is increasingly required for understanding and engineering biological systems. Small-molecule-responsive transcriptional regulation has been widely used to control transgene expression. In contrast, methods for specific and simultaneous regulation of multiple genes with a single regulatory protein remain undeveloped. In this report, we describe a method for quantitatively tuning the expression of multiple transgenes with a translational regulatory protein. A protein that binds a specific RNA motif inserted in the 5'-untranslated region (UTR) of an mRNA modulates the translation of that message in mammalian cells. We provide two independent mechanisms by which to rationally fine-tune the output: the efficiency of translation correlates well with the distance between the inserted motif and the 5' terminus of the mRNA and is further modulated by the tandem insertion of multiple RNA motifs. The combination of these two approaches allowed us to fine-tune the translational efficiency of target mRNAs over a wide dynamic range. Moreover, we controlled the expression of two transgenes simultaneously and specifically by engineering each cis-regulatory 5'-UTR. The approach provides a useful alternative regulatory layer for controlling gene expression in biological research and engineering.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.