ArticleEuropean journal of medical research2013
Roles of virulence genes (PsaA and CpsA) on the invasion of Streptococcus pneumoniae into blood system.
Article in European journal of medical research, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.
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Who cites it
6 citing papers in PubMed.
- Pangenome analysis and virulence profiling of Streptococcus intermedius.BMC genomics · 2021Article
- Molecular characterization, antibiotic resistance pattern and capsular types of invasive Streptococcus pneumoniae isolated from clinical samples in Tehran, Iran.BMC microbiology · 2020Article
- Impact of different Streptococcus pneumoniae on the secretion of interleukin and adhesin from THP-1 monocytes.Journal of clinical laboratory analysis · 2019Article
- Identifying genes associated with invasive disease in S. pneumoniae by applying a machine learning approach to whole genome sequence typing data.Scientific reports · 2019Article
- In vitro expression of Streptococcus pneumoniae ply gene in human monocytes and pneumocytes.European journal of medical research · 2015Article
- Genome-wide identification of genes essential for the survival of Streptococcus pneumoniae in human saliva.PloS one · 2014Article
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11 authors.
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Abstract
backgroundStreptococcus pneumoniae (SP) is the major cause of childhood mortality worldwide, we need to understand virulence genes of SP so can better target the treatment.We investigated the expression of virulence genes PsaA and CpsA in different strains of SP interacting with monocyte cell line (THP-1) or pneumocyte cell line (A549) and the possible mechanism of SP invasion of the blood system.
methodsA total of 23 strains of SP were collected from hospitalized patients (blood-derived and sputum-derived) in the Second Affiliated Hospital of Wenzhou Medical College. The strains and ATCC 49619 were cultured, and RNAs were extracted. THP-1 and A549 cells were stimulated by different SP and ATCC 49619 for 4 h and 8 h, respectively. Quantitative real-time PCR was used to analyze the mRNA expression of PsaA and CpsA. The data were analyzed by SPSS 17.0.
resultsThe mRNA level of PsaA and CpsA were all significantly increased in clinical SP strains when compared to ATCC49619 after tedTHP-1 and A549 cells were stimulated. Clinical SPs showed higher virulence compared with ATCC49619.
conclusionsThe expression of CpsA is the basis of the pathogenicity of SP. The expression of virulence gene PsaA may be helpful to the invasion of SP to the blood system.
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