ArticleAnalytical chemistry2013
Analysis of selected sugars and sugar phosphates in mouse heart tissue by reductive amination and liquid chromatography-electrospray ionization mass spectrometry.
Article in Analytical chemistry, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed, 53 citations in OpenAlex.
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- PI3K-dependent reprogramming of hexokinase isoforms controls glucose metabolism and functional responses of B lymphocytes.iScience · 2024Article
- Coordinated Regulation of Central Carbon Metabolism in Pyroligneous Acid-Treated Tomato Plants under Aluminum Stress.Metabolites · 2023Article
- Inhibition of mTOR improves malnutrition induced hepatic metabolic dysfunction.Scientific reports · 2022Article
- Effects of water quality on growth performance and health of nursery pigs.Translational animal science · 2022Article
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- Progress and Challenges in Quantifying Carbonyl-Metabolomic Phenomes with LC-MS/MS.Molecules (Basel, Switzerland) · 2021Review
- Mass Spectrometry-based Metabolomics in Translational Research.Advances in experimental medicine and biology · 2021Review
- Structure and Function ofACS omega · 2020Article
- First characterization of glucose flux through the hexosamine biosynthesis pathway (HBP) inThe Journal of biological chemistry · 2020Article
- Intense Light-Mediated Circadian Cardioprotection via Transcriptional Reprogramming of the Endothelium.Cell reports · 2019Article
- ADP heptose, a novel pathogen-associated molecular pattern identified inFASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2019Article
- Glycation of Plant Proteins: Regulatory Roles and Interplay with Sugar Signalling?International journal of molecular sciences · 2019Review
- Two-step derivatization for determination of sugar phosphates in plants by combined reversed phase chromatography/tandem mass spectrometry.Plant methods · 2019Article
- Acetyl Phosphate as a Primordial Energy Currency at the Origin of Life.Origins of life and evolution of the biosphere : the journal of the International Society for the Study of the Origin of Life · 2018Article
- A versatile LC-MS/MS approach for comprehensive, quantitative analysis of central metabolic pathways.Wellcome open research · 2018Article
- Cardiac Ryanodine Receptor (Ryr2)-mediated Calcium Signals Specifically Promote Glucose Oxidation via Pyruvate Dehydrogenase.The Journal of biological chemistry · 2016Article
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 2 countries.
Funding
Abstract
Sensitive and reliable analysis of sugars and sugar phosphates in tissues and cells is essential for many biological and cell engineering studies. However, the successful analysis of these endogenous compounds in biological samples by liquid chromatography/electrospray ionization mass spectrometry (LC/ESI-MS) is often difficult because of their poor chromatographic retention properties in reversed-phase LC, the complex biological matrices, and the ionization suppression in ESI. This situation is further complicated by the existence of their multiple structural isomers in vivo. This work describes the combination of reductive amination using 3-amino-9-ethylcarbazole, with a new LC approach using a pentafluorophenyl core-shell ultrahigh performance (UP) LC column and methylphosphonic acid as an efficient tail-sweeping reagent for improved chromatographic separation. This new method was used for selected detection and accurate quantitation of the major free and phosphorylated reducing sugars in mouse heart tissue. Among the detected compounds, accurate quantitation of glyceraldehyde, ribose, glucose, glycerylaldehyde-3-phosphate, ribose-5-phosphate, glucose-6-phosphate, and mannose-6-phosphate was achieved by UPLC/multiple-reaction monitoring (MRM)-MS, with analytical accuracies ranging from 87.4% to 109.4% and CVs of ≤8.5% (n = 6). To demonstrate isotope-resolved metabolic profiling, we used UPLC/quadrupole time-of-flight (QTOF)-MS to analyze the isotope distribution patterns of C3 to C6 free and phosphorylated reducing sugars in heart tissues from (13)C-labeled wild type and knockout mice. In conclusion, the preanalytical derivatization-LC/ESI-MS method has resulted in selective determination of free and phosphorylated reducing sugars without the interferences from their nonreducing structural isomers in mouse heart tissue, with analytical sensitivities in the femtomole to low picomole range.
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