ArticleWorld journal of microbiology & biotechnology2013
Enhanced morphinan alkaloid production in hairy root cultures of Papaver bracteatum by over-expression of salutaridinol 7-o-acetyltransferase gene via Agrobacterium rhizogenes mediated transformation.
Article in World journal of microbiology & biotechnology, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
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11 citing papers in PubMed, 44 citations in OpenAlex.
- Reprogramming Hairy Root Cultures: A Synthetic Biology Framework for Precision Metabolite Biosynthesis.Plants (Basel, Switzerland) · 2025Review
- The Untapped Potential of Hairy Root Cultures and Their Multiple Applications.International journal of molecular sciences · 2024Review
- Effect of silver nanoparticles on tropane alkaloid production of transgenic hairy root cultures of Hyoscyamus muticus L. and their antimicrobial activity.Scientific reports · 2023Article
- ARPI, β-AS, and UGE regulate glycyrrhizin biosynthesis in Glycyrrhiza uralensis hairy roots.Plant cell reports · 2021Article
- Evaluation of Polyphenolic Compounds and Pharmacological Activities in Hairy Root Cultures ofMolecules (Basel, Switzerland) · 2019Article
- Article
- Green Routes for the Production of Enantiopure Benzylisoquinoline Alkaloids.International journal of molecular sciences · 2017Review
- Analysis of propagation of Bacopa monnieri (L.) from hairy roots, elicitation and Bacoside A contents of Ri transformed plants.World journal of microbiology & biotechnology · 2016Article
- In vitro regeneration and Agrobacterium mediated genetic transformation of Artemisia aucheri Boiss.Physiology and molecular biology of plants : an international journal of functional plant biology · 2014Article
- Hairy root induction and plant regeneration of medicinal plant Dracocephalum kotschyi.Physiology and molecular biology of plants : an international journal of functional plant biology · 2014Article
- Enhanced production of hyoscyamine and scopolamine from genetically transformed root culture ofIn vitro cellular & developmental biology. Plant : journal of the Tissue Culture AssociationArticle
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Authors and funding
6 authors at 3 institutions in 1 country.
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Abstract
Papaver bracteatum is an important medicinal plant valued for its high content of thebaine and an alternative to P. somniferum for benzylisoquinoline alkaloid production. Salutaridinol 7-o-acetyltransferase (SalAT) is a key gene in morphinan alkaloids biosynthesis pathway. Over expression of SalAT gene was used for metabolic engineering in P. bracteatum hairy root cultures. Transcript level of the salutaridinol 7-o-acetyltransferase gene in transgenic hairy root lines increased up to 154 and 128 % in comparison with hairy roots without SalAT over expression and wild type roots, respectively. High performance liquid chromatography analysis showed that the transgenic hairy roots relatively improved levels of thebaine (1.28 % dry weight), codeine (0.02 % dry weight) and morphine (0.03 % dry weight) compared to those hairy roots without SalAT over expression. This suggests that P. bracteatum hairy roots expressing the SalAT gene could be potentially used for the production of valuable morphinan alkaloids.
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