ArticleDaru : journal of Faculty of Pharmacy, Tehran University of Medical Sciences2013
Cytotoxic evaluation of Melia azedarach in comparison with, Azadirachta indica and its phytochemical investigation.
Article in Daru : journal of Faculty of Pharmacy, Tehran University of Medical Sciences, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers, 1 of them a synthesis that pooled it.
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Who cites it
12 citing papers in PubMed, 1 synthesis or guideline pooled it.
- A Review of Cytotoxic Plants of the Indian Subcontinent and a Broad-Spectrum Analysis of Their Bioactive Compounds.Molecules (Basel, Switzerland) · 2020Pooled it
- A Comparative Study on the Antidiabetic Activity, Cytotoxicity and Lipid Profile ofPlants (Basel, Switzerland) · 2024Article
- Improvement and prediction of the extraction parameters of lupeol and stigmasterol metabolites of Melia azedarach with response surface methodology.BMC biotechnology · 2024Article
- Antibacterial Properties of Methanolic Leaf Extracts ofMicroorganisms · 2023Article
- Evaluation of Chemical Compositions and the Antioxidant and Cytotoxic Properties of the Aqueous Extract of Tri-Yannarose Recipe (Antioxidants (Basel, Switzerland) · 2023Article
- Pregnane Steroids from the Leaves of Melia Azedarach and Apoptotic Activity against T47D Cells.Asian Pacific journal of cancer prevention : APJCP · 2021Article
- Bio-selective hormonal breast cancer cytotoxic and antioxidant potencies ofBiotechnology reports (Amsterdam, Netherlands) · 2020Article
- Chemical Constituents ofTurkish journal of pharmaceutical sciences · 2018Article
- The anti-tubercular activity ofAsian Pacific journal of tropical biomedicine · 2016Article
- Screening of Korean Natural Products for Anti-Adipogenesis Properties and Isolation of Kaempferol-3-O-rutinoside as a Potent Anti-Adipogenetic Compound from Solidago virgaurea.Molecules (Basel, Switzerland) · 2016Article
- Neem components as potential agents for cancer prevention and treatment.Biochimica et biophysica acta · 2014Review
- Article
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Authors and funding
7 authors.
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Abstract
backgroundMelia azedarach L. is an important medicinal plant that is used for variety of ailments in Iranian traditional medicine. Azadirachta indica A. Juss is its allied species and possesses similar properties and effects. The present study was undertaken to investigate anticancer activity of these M. azedarach in comparison with A. indica on cancer cell lines and also to evaluate their safety in humans by testing them on normal cell line. The study also aimed to determine the active components that are responsible for medicinal effects of M. azedarach in traditional usages.
methodsIn this study, the cytotoxic activity of crude extracts from M. azedarach and A. indica leaves, pulps and seeds as well as three main fractions of their leaf extracts were assayed against HT-29, A-549, MCF-7 and HepG-2 and MDBK cell lines. MTT assay was used to evaluate their cytotoxic activities. Methanol leaf fraction of M. azedarach as the safest leaf fraction in terms of cytotoxicity was subjected for phytochemical study.
resultsResults of the present study indicated that seed kernel extract of M. azedarach had the highest cytotoxic activity and selectivity to cancer cell lines (IC50 range of 8.18- 60.10 μg mL-1). In contrast to crude seed extract of A. indica, crude pulp and crude leaf extracts of this plant showed remarkably stronger anti-prolifrative activity (IC50 ranges of 83.45 - 212.16 μg mL-1 and 34.11- 95.51 μg mL-1 respectively) than those of M. azedarach (all IC50 values of both plants > 650 μg mL-1). The phytochemical analysis led to the isolation of four flavonol 3-O-glycosides including rutin, kaempferol-3-O-robinobioside, kaempferol-3-O-rutinoside and isoquercetin along with a purin nucleoside, β-adenosine.
conclusionsThe anti-prolifrative potentials of extracts from different parts of M. azedarach and A. indica were determined. By comparison, methanol leaf fraction of M. azedarach seems to be safer in terms of cytotoxicity. Our study shows that flavonols are abundant in the leaves of M. azedarach and these compounds seem to be responsible for many of medicinal effects exploited in the traditional uses.
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