ArticleBMC plant biology2013
Transcriptome resources and functional characterization of monoterpene synthases for two host species of the mountain pine beetle, lodgepole pine (Pinus contorta) and jack pine (Pinus banksiana).
Article in BMC plant biology, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 31 papers.
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31 citing papers in PubMed, 60 citations in OpenAlex.
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- Plasticity engineering of plant monoterpene synthases and application for microbial production of monoterpenoids.Biotechnology for biofuels · 2021Review
- Characterization and Analysis of the Full-Length Transcriptomes of Multiple Organs inInternational journal of molecular sciences · 2020Article
- On the Evolution and Functional Diversity of Terpene Synthases in the Pinus Species: A Review.Journal of molecular evolution · 2020Review
- Linking genotype to phenotype to identify genetic variation relating to host susceptibility in the mountain pine beetle system.Evolutionary applications · 2020Article
- Functional and morphological evolution in gymnosperms: A portrait of implicated gene families.Evolutionary applications · 2020Article
- Article
- The cytochrome P450 CYP6DE1 catalyzes the conversion of α-pinene into the mountain pine beetle aggregation pheromone trans-verbenol.Scientific reports · 2019Article
- Cytochromes P450 Preferentially Expressed in Antennae of the Mountain Pine Beetle.Journal of chemical ecology · 2019Article
- Article
- Drought-induced susceptibility for Cenangium ferruginosum leads to progression of Cenangium-dieback disease in Pinus koraiensis.Scientific reports · 2018Article
- Cyanobacterial production of plant essential oils.Planta · 2018Article
Corrections and comments
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Authors and funding
16 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundThe mountain pine beetle (MPB, Dendroctonus ponderosae) epidemic has affected lodgepole pine (Pinus contorta) across an area of more than 18 million hectares of pine forests in western Canada, and is a threat to the boreal jack pine (Pinus banksiana) forest. Defence of pines against MPB and associated fungal pathogens, as well as other pests, involves oleoresin monoterpenes, which are biosynthesized by families of terpene synthases (TPSs). Volatile monoterpenes also serve as host recognition cues for MPB and as precursors for MPB pheromones. The genes responsible for terpene biosynthesis in jack pine and lodgepole pine were previously unknown.
resultsWe report the generation and quality assessment of assembled transcriptome resources for lodgepole pine and jack pine using Sanger, Roche 454, and Illumina sequencing technologies. Assemblies revealed transcripts for approximately 20,000 - 30,000 genes from each species and assembly analyses led to the identification of candidate full-length prenyl transferase, TPS, and P450 genes of oleoresin biosynthesis. We cloned and functionally characterized, via expression of recombinant proteins in E. coli, nine different jack pine and eight different lodgepole pine mono-TPSs. The newly identified lodgepole pine and jack pine mono-TPSs include (+)-α-pinene synthases, (-)-α-pinene synthases, (-)-β-pinene synthases, (+)-3-carene synthases, and (-)-β-phellandrene synthases from each of the two species.
conclusionIn the absence of genome sequences, transcriptome assemblies are important for defence gene discovery in lodgepole pine and jack pine, as demonstrated here for the terpenoid pathway genes. The product profiles of the functionally annotated mono-TPSs described here can account for the major monoterpene metabolites identified in lodgepole pine and jack pine.
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