ArticleNano letters2013
Translocating kilobase RNA through the Staphylococcal α-hemolysin nanopore.
Article in Nano letters, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
23 citing papers in PubMed.
- Spontaneous DNA translocation through a van der Waals heterostructure nanopore for single-molecule detection.Nanoscale advances · 2021Article
- Recent advances in biological nanopores for nanopore sequencing, sensing and comparison of functional variations in MspA mutants.RSC advances · 2021Review
- Biological Nanopores: Engineering on Demand.Life (Basel, Switzerland) · 2021Review
- Modulation of Photoinduced Transmembrane Currents in a Fullerene-Doped Freestanding Lipid Bilayer by a Lateral Bias.ACS omega · 2019Article
- Polarization Induced Electro-Functionalization of Pore Walls: A Contactless Technology.Biosensors · 2019Review
- If Squeezed, a Camel Passes Through the Eye of a Needle: Voltage-Mediated Stretching of Dendrimers Facilitates Passage Through a Nanopore.The Journal of membrane biology · 2018Article
- Electro-osmotic capture and ionic discrimination of peptide and protein biomarkers with FraC nanopores.Nature communications · 2017Article
- Nanoscale Investigation of Generation 1 PAMAM Dendrimers Interaction with a Protein Nanopore.Scientific reports · 2017Article
- Single-cell RNA-sequencing: The future of genome biology is now.RNA biology · 2017Review
- Electrophoretic Deformation of Individual Transfer RNA Molecules Reveals Their Identity.Nano letters · 2016Article
- Mimicking Ribosomal Unfolding of RNA Pseudoknot in a Protein Channel.Journal of the American Chemical Society · 2015Article
- A Protein Rotaxane Controls the Translocation of Proteins Across a ClyA Nanopore.Nano letters · 2015Article
- DNA/RNA transverse current sequencing: intrinsic structural noise from neighboring bases.Frontiers in genetics · 2015Article
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- Internal vs fishhook hairpin DNA: unzipping locations and mechanisms in the α-hemolysin nanopore.The journal of physical chemistry. B · 2014Article
- Single-cell RNA-seq: advances and future challenges.Nucleic acids research · 2014Review
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- Detection of 3'-end RNA uridylation with a protein nanopore.ACS nano · 2014Article
- The evolution of nanopore sequencing.Frontiers in genetics · 2014Review
Corrections and comments
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Authors and funding
3 authors.
Funding
Abstract
The electrophoretic translocation of polynucleotides through nanopores may permit direct single-molecule nucleic acid sequencing. Here we describe the translocation of ssRNA heteropolymers (91-6083 bases) through the α-hemolysin nanopore. Translocation of these long ssRNAs is characterized by surprisingly long, almost complete ionic current blockades with durations averaging milliseconds per base (at +180 mV). The event durations decrease exponentially with increased transmembrane potential but are largely unaffected by the presence of urea. When the ssRNA is coupled at the 3' end to streptavidin, which cannot translocate through the pore, permanent blockades are observed, supporting our conclusion that the transient blockades arise from ssRNA translocation.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.