ArticleAnalytical chemistry2013
Nanofluidic platform for single mitochondria analysis using fluorescence microscopy.
Article in Analytical chemistry, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed.
- Light Scattering Contrast Inversion of Single Metal Nanoparticles Inside a Nanofluidic Channel.The journal of physical chemistry. C, Nanomaterials and interfaces · 2026Article
- Super-Resolution Imaging of Voltages in the Interior of Individual, Vital Mitochondria.ACS nano · 2024Article
- Photobleaching and phototoxicity of mitochondria in live cell fluorescent super-resolution microscopy.Mitochondrial communications · 2024Article
- Thioflavin T indicates mitochondrial membrane potential in mammalian cells.Biophysical reports · 2023Article
- Future of Digital Assays to Resolve Clinical Heterogeneity of Single Extracellular Vesicles.ACS nano · 2022Review
- Micro- and Nano-Devices for Studying Subcellular Biology.Small (Weinheim an der Bergstrasse, Germany) · 2021Review
- A Review of Secondary Flow in Inertial Microfluidics.Micromachines · 2020Review
- Single-Molecule Detection of DNA in a Nanochannel by High-Field Strength-Assisted Electrical Impedance Spectroscopy.Micromachines · 2019Article
- Temporal metabolic partitioning of the yeast and protist cellular networks: the cell is a global scale-invariant (fractal or self-similar) multioscillator.Journal of biomedical optics · 2018Article
- Mitochondria, Bioenergetics and Apoptosis in Cancer.Trends in cancer · 2017Review
- Resistive flow sensing of vital mitochondria with nanoelectrodes.Mitochondrion · 2017Article
- High-Throughput Inertial Focusing of Micrometer- and Sub-Micrometer-Sized Particles Separation.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2017Article
- The rise of mitochondria in medicine.Mitochondrion · 2016Review
- Nanohole Array-Directed Trapping of Mammalian Mitochondria Enabling Single Organelle Analysis.Analytical chemistry · 2015Article
- Simultaneous measurement of individual mitochondrial membrane potential and electrophoretic mobility by capillary electrophoresis.Analytical chemistry · 2014Article
- Micro total analysis systems: fundamental advances and biological applications.Analytical chemistry · 2014Review
Corrections and comments
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Authors and funding
5 authors.
Funding
Abstract
Using nanofluidic channels in PDMS of cross section 500 nm × 2 μm, we demonstrate the trapping and interrogation of individual, isolated mitochondria. Fluorescence labeling demonstrates the immobilization of mitochondria at discrete locations along the channel. Interrogation of mitochondrial membrane potential with different potential sensitive dyes (JC-1 and TMRM) indicates the trapped mitochondria are vital in the respiration buffer. Fluctuations of the membrane potential can be observed at the single mitochondrial level. A variety of chemical challenges can be delivered to each individual mitochondrion in the nanofluidic system. As sample demonstrations, increases in the membrane potential are seen upon introduction of OXPHOS substrates into the nanofluidic channel. Introduction of Ca(2+) into the nanochannels induces mitochondrial membrane permeabilization (MMP), leading to depolarization, observed at the single mitochondrial level. A variety of applications in cancer biology, stem cell biology, apoptosis studies, and high throughput functional metabolomics studies can be envisioned using this technology.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.