Evidence map›Paper›PMID 23678074›Full record

ArticleJournal of clinical microbiology2013

Identification of medically relevant species of arthroconidial yeasts by use of matrix-assisted laser desorption ionization-time of flight mass spectrometry.

Anna Kolecka, Kantarawee Khayhan, Marizeth Groenewald, Bart Theelen, Michael Arabatzis, Aristea Velegraki, Markus Kostrzewa, Mihai Mares, Saad J Taj-Aldeen, Teun Boekhout

Open access · bronzeAbstract readEvaluation Study
In one paragraph

Article in Journal of clinical microbiology, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 43 papers.

0numbers the graph read from it
0cells of the map it votes in
43citing papers in PubMed
5.1field-weighted citation impact, top 4% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

43 citing papers in PubMed, 99 citations in OpenAlex.

  1. Targeted Screening to PredictJournal of fungi (Basel, Switzerland) · 2026
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  3. An unexpected opportunist:Medical mycology case reports · 2024
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  6. Saprochaete/Magnusiomyces: identification, virulence factors, and antifungal susceptibility of a challenging rare yeast.Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology] · 2024
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  9. Mass spectrometry in research laboratories and clinical diagnostic: a new era in medical mycology.Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology] · 2022
    Review
  10. Article
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  12. Review
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  14. Review
  15. Current medical mycology · 2020
    Article
  16. InvasivePathogens (Basel, Switzerland) · 2020
    Review
  17. Answer to November 2020 Photo Quiz.Journal of clinical microbiology · 2020
    Article
  18. Article
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  20. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors at 5 institutions in 7 countries.

Anna KoleckaCBS-KNAW Fungal Biodiversity Centre, Utrecht, the Netherlands.
Kantarawee Khayhan
Marizeth Groenewald
Bart Theelen
Michael Arabatzis
Aristea Velegraki
Markus Kostrzewa
Mihai Mares
Saad J Taj-Aldeen
Teun Boekhout
Westerdijk Fungal Biodiversity Institute · NLNational and Kapodistrian University of Athens · GRBruker (Germany) · DEHamad Medical Corporation · QA"Ion Ionescu de la Brad" Iasi University of Life Sciences · RO

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) was used for an extensive identification study of arthroconidial yeasts, using 85 reference strains from the CBS-KNAW yeast collection and 134 clinical isolates collected from medical centers in Qatar, Greece, and Romania. The test set included 72 strains of ascomycetous yeasts (Galactomyces, Geotrichum, Saprochaete, and Magnusiomyces spp.) and 147 strains of basidiomycetous yeasts (Trichosporon and Guehomyces spp.). With minimal preparation time, MALDI-TOF MS proved to be an excellent diagnostic tool that provided reliable identification of most (98%) of the tested strains to the species level, with good discriminatory power. The majority of strains were correctly identified at the species level with good scores (>2.0) and seven of the tested strains with log score values between 1.7 and 2.0. The MALDI-TOF MS results obtained were consistent with validated internal transcribed spacer (ITS) and/or large subunit (LSU) ribosomal DNA sequencing results. Expanding the mass spectrum database by increasing the number of reference strains for closely related species, including those of nonclinical origin, should enhance the usefulness of MALDI-TOF MS-based diagnostic analysis of these arthroconidial fungi in medical and other laboratories.

Indexed as

Clinical Laboratory TechniquesGreeceHumansMycologyMycosesQatarRomaniaSensitivity and SpecificitySpecimen HandlingSpectrometry, Mass, Matrix-Assisted Laser Desorption-IonizationTime FactorsYeasts

Identifiers

PMID23678074
PMCPMC3719645
OpenAlexW2114648815

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.