ArticleJournal of clinical microbiology2013
Detection of Mycobacterium tuberculosis in blood by use of the Xpert MTB/RIF assay.
Article in Journal of clinical microbiology, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed, 28 citations in OpenAlex.
- Diagnostic accuracy and inter-lot precision of the FujiLAM II point-of-care assay to detect tuberculosis in stored urine specimens from people living with HIV.Microbiology spectrum · 2026Article
- Whole Blood vs Serum-Derived Exosomes for Host and Pathogen-Specific Tuberculosis Biomarker Identification: RNA-Seq-Based Machine-Learning Approach.Biochemical genetics · 2025Article
- Xpert MTB/RIF Ultra enables sensitive detection ofThe international journal of tuberculosis and lung disease : the official journal of the International Union against Tuberculosis and Lung Disease · 2024Article
- Difficulties with the implemented xpert MTB/RIF for determining diagnosis of pulmonary and extrapulmonary tuberculosis in adults and children.Journal of clinical tuberculosis and other mycobacterial diseases · 2020Article
- Multiplex Detection of Three Select Agents Directly from Blood by Use of the GeneXpert System.Journal of clinical microbiology · 2019Article
- Article
- Rapid Detection of Bacillus anthracis Bloodstream Infections by Use of a Novel Assay in the GeneXpert System.Journal of clinical microbiology · 2017Article
- Disseminated tuberculosis among hospitalised HIV patients in South Africa: a common condition that can be rapidly diagnosed using urine-based assays.Scientific reports · 2017Article
- Rapid culture-based detection of living mycobacteria using microchannel electrical impedance spectroscopy (m-EIS).Biological research · 2017Article
- Evaluation of the Xpert® MTB/RIF assay and microscopy for the diagnosis of Mycobacterium tuberculosis in Namibia.Infectious diseases of poverty · 2017Article
- Sensitive Detection of Francisella tularensis Directly from Whole Blood by Use of the GeneXpert System.Journal of clinical microbiology · 2017Article
- The role of Xpert MTB/RIF in diagnosing pulmonary tuberculosis in post-mortem tissues.Scientific reports · 2016Article
- A Novel Sample Processing Method for Rapid Detection of Tuberculosis in the Stool of Pediatric Patients Using the Xpert MTB/RIF Assay.PloS one · 2016Article
- Economic challenges associated with tuberculosis diagnostic development.Expert review of pharmacoeconomics & outcomes research · 2014Review
- Determinants of PCR performance (Xpert MTB/RIF), including bacterial load and inhibition, for TB diagnosis using specimens from different body compartments.Scientific reports · 2014Article
- Article
- Diagnostic accuracy of Xpert MTB/RIF for extrapulmonary tuberculosis specimens: establishing a laboratory testing algorithm for South Africa.Journal of clinical microbiology · 2014Article
- Exploring alternative biomaterials for diagnosis of pulmonary tuberculosis in HIV-negative patients by use of the GeneXpert MTB/RIF assay.Journal of clinical microbiology · 2013Article
- Evaluation of Xpert MTB/RIF for detection of tuberculosis from blood samples of HIV-infected adults confirms Mycobacterium tuberculosis bacteremia as an indicator of poor prognosis.Journal of clinical microbiology · 2013Article
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
We have developed a novel blood lysis-centrifugation approach for highly sensitive Mycobacterium tuberculosis detection in large volumes of blood with the Xpert MTB/RIF assay. One through 20 ml of blood was spiked with 0.25 to 10 CFU/ml of the M. tuberculosis surrogate M. bovis BCG. Multiple replicates of each sample were processed by a new lysis-centrifugation method and tested with the Xpert MTB/RIF assay. The assay was very sensitive with increased blood volumes. In the 20-ml samples, BCG was detected in blood spiked with 10, 5, 1, and 0.25 CFU/ml 100, 100, 83, and 57% of the time, respectively, compared to 100, 66, 18, and 18%, of the time, respectively, in 1-ml blood samples. Assay sensitivity was influenced by the type of anticoagulant used, with acid-citrate-dextrose solution B (ACD-B) providing the best results. A limit of detection of 10 CFU/ml was established with BCG spiked into ACD-B-treated blood, and 92, 36, and 33% of the samples with 5, 1, and 0.5 CFU/ml, respectively, were assay positive. The lysis buffer was stable both at room temperature and at 4°C for 2 months. The assay was tested with blood stored for 8 days without a change in sensitivity as measured by cycle threshold. This new assay format extends the capability of the Xpert MTB/RIF test, enabling up to 20 ml of blood to be tested rapidly for the presence of M. tuberculosis. This approach may be a useful method to detect extrapulmonary tuberculosis and the risk of death in immunocompromised patients.
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