ArticleJournal of clinical microbiology2013
Subtyping of Salmonella enterica serovar Newport outbreak isolates by CRISPR-MVLST and determination of the relationship between CRISPR-MVLST and PFGE results.
Article in Journal of clinical microbiology, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed, 72 citations in OpenAlex.
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- Longitudinal study highlights patterns ofFrontiers in microbiology · 2025Article
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- Genomic and phenotypic characterization ofMicrobial genomics · 2023Article
- Comprehensive Assessment of Subtyping Methods for Improved Surveillance of Foodborne Salmonella.Microbiology spectrum · 2022Article
- Antimicrobial Resistance and CRISPR Typing AmongFrontiers in microbiology · 2021Article
- High similarity and high frequency of virulence genes among Salmonella Dublin strains isolated over a 33-year period in Brazil.Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology] · 2020Article
- Assessment and Comparison of Molecular Subtyping and Characterization Methods forFrontiers in microbiology · 2019Review
- Commentary: Study the Features of 57 Confirmed CRISPR Loci in 38 Strains ofFrontiers in microbiology · 2019Article
- High-Resolution Identification of Multiple Salmonella Serovars in a Single Sample by Using CRISPR-SeroSeq.Applied and environmental microbiology · 2018Article
- Study the Features of 57 Confirmed CRISPR Loci in 38 Strains ofFrontiers in microbiology · 2018Article
- Pathogenomics of Virulence Traits ofFrontiers in microbiology · 2018Article
- A decade of discovery: CRISPR functions and applications.Nature microbiology · 2017Review
- Metagenomic reconstructions of bacterial CRISPR loci constrain population histories.The ISME journal · 2016Article
- Allelic variation contributes to bacterial host specificity.Nature communications · 2015Article
- Comparative analysis of subtyping methods against a whole-genome-sequencing standard for Salmonella enterica serotype Enteritidis.Journal of clinical microbiology · 2015Article
- High-Throughput Sequencing, a VersatileWeapon to Support Genome-Based Diagnosis in Infectious Diseases: Applications to Clinical Bacteriology.Pathogens (Basel, Switzerland) · 2014Review
- The role of CRISPR-Cas systems in virulence of pathogenic bacteria.Microbiology and molecular biology reviews : MMBR · 2014Review
- Lactobacillus buchneri genotyping on the basis of clustered regularly interspaced short palindromic repeat (CRISPR) locus diversity.Applied and environmental microbiology · 2014Article
- CRISPRs: molecular signatures used for pathogen subtyping.Applied and environmental microbiology · 2014Review
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Authors and funding
6 authors at 4 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Salmonella enterica subsp. enterica serovar Newport (S. Newport) is the third most prevalent cause of food-borne salmonellosis. Rapid, efficient, and accurate methods for identification are required to track specific strains of S. Newport during outbreaks. By exploiting the hypervariable nature of virulence genes and clustered regularly interspaced short palindromic repeats (CRISPRs), we previously developed a sequence-based subtyping approach, designated CRISPR-multi-virulence-locus sequence typing (CRISPR-MVLST). To demonstrate the applicability of this approach, we analyzed a broad set of S. Newport isolates collected over a 5-year period by using CRISPR-MVLST and pulsed-field gel electrophoresis (PFGE). Among 84 isolates, we defined 38 S. Newport sequence types (NSTs), all of which were novel compared to our previous analyses, and 62 different PFGE patterns. Our data suggest that both subtyping approaches have high discriminatory abilities (>0.95) with a potential for clustering cases with common exposures. Importantly, we found that isolates from closely related NSTs were often similar by PFGE profile as well, further corroborating the applicability of CRISPR-MVLST. In the first full application of CRISPR-MVLST, we analyzed isolates from a recent S. Newport outbreak. In this blinded study, we confirmed the utility of CRISPR-MVLST and were able to distinguish the 10 outbreak isolates, as defined by PFGE and epidemiological data, from a collection of 20 S. Newport isolates. Together, our data show that CRISPR-MVLST could be a complementary approach to PFGE subtyping for S. Newport.
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