ArticleThe Journal of biological chemistry2013
High specificity in CheR methyltransferase function: CheR2 of Pseudomonas putida is essential for chemotaxis, whereas CheR1 is involved in biofilm formation.
Article in The Journal of biological chemistry, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed.
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- Metatranscriptomics Uncover Diurnal Functional Shifts in Bacterial Transgenes with Profound Metabolic Effects.Cell host & microbe · 2025Article
- Plant Growth-Promoting Effect and Complete Genomic Sequence Analysis of the Beneficial RhizosphereMicroorganisms · 2025Article
- Effects of genetic and environmental variables on biofilm development dynamics inIranian journal of microbiology · 2023Article
- Review
- Atypical chemoreceptor arrays accommodate high membrane curvature.Nature communications · 2020Article
- Concentration Dependent Effect of Plant Root Exudates on the Chemosensory Systems ofFrontiers in microbiology · 2019Article
- Protein Activity Sensing in Bacteria in Regulating Metabolism and Motility.Frontiers in microbiology · 2019Review
- Molecular Basis for Autocatalytic Backbone N-Methylation in RiPP Natural Product Biosynthesis.ACS chemical biology · 2018Article
- The activity of the C4-dicarboxylic acid chemoreceptor of Pseudomonas aeruginosa is controlled by chemoattractants and antagonists.Scientific reports · 2018Article
- Structural analyses unravel the molecular mechanism of cyclic di-GMP regulation of bacterial chemotaxis via a PilZ adaptor protein.The Journal of biological chemistry · 2018Article
- Sensory Repertoire of Bacterial Chemoreceptors.Microbiology and molecular biology reviews : MMBR · 2017Review
- Assigning chemoreceptors to chemosensory pathways inProceedings of the National Academy of Sciences of the United States of America · 2017Article
- The expression of many chemoreceptor genes depends on the cognate chemoeffector as well as on the growth medium and phase.Current genetics · 2017Article
- Metabolic Value Chemoattractants Are Preferentially Recognized at Broad Ligand Range Chemoreceptor ofFrontiers in microbiology · 2017Article
- Pseudomonas aeruginosa EftM Is a Thermoregulated Methyltransferase.The Journal of biological chemistry · 2016Article
- Genetic Dissection of the Regulatory Network Associated with High c-di-GMP Levels in Pseudomonas putida KT2440.Frontiers in microbiology · 2016Article
- Determining Roles of Accessory Genes in Denitrification by Mutant Fitness Analyses.Applied and environmental microbiology · 2016Article
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7 authors.
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No grant is acknowledged in the PubMed record.
Abstract
Chemosensory pathways are a major signal transduction mechanism in bacteria. CheR methyltransferases catalyze the methylation of the cytosolic signaling domain of chemoreceptors and are among the core proteins of chemosensory cascades. These enzymes have primarily been studied Escherichia coli and Salmonella typhimurium, which possess a single CheR involved in chemotaxis. Many other bacteria possess multiple cheR genes. Because the sequences of chemoreceptor signaling domains are highly conserved, it remains to be established with what degree of specificity CheR paralogues exert their activity. We report here a comparative analysis of the three CheR paralogues of Pseudomonas putida. Isothermal titration calorimetry studies show that these paralogues bind the product of the methylation reaction, S-adenosylhomocysteine, with much higher affinity (KD of 0.14-2.2 μM) than the substrate S-adenosylmethionine (KD of 22-43 μM), which indicates product feedback inhibition. Product binding was particularly tight for CheR2. Analytical ultracentrifugation experiments demonstrate that CheR2 is monomeric in the absence and presence of S-adenosylmethionine or S-adenosylhomocysteine. Methylation assays show that CheR2, but not the other paralogues, methylates the McpS and McpT chemotaxis receptors. The mutant in CheR2 was deficient in chemotaxis, whereas mutation of CheR1 and CheR3 had either no or little effect on chemotaxis. In contrast, biofilm formation of the CheR1 mutant was largely impaired but not affected in the other mutants. We conclude that CheR2 forms part of a chemotaxis pathway, and CheR1 forms part of a chemosensory route that controls biofilm formation. Data suggest that CheR methyltransferases act with high specificity on their cognate chemoreceptors.
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