ArticleMolecular and cellular biochemistry2013
Promotion of autophagy at the maturation step by IL-6 is associated with the sustained mitogen-activated protein kinase/extracellular signal-regulated kinase activity.
Article in Molecular and cellular biochemistry, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed, 28 citations in OpenAlex.
- Matrine induces V-ATPase-dependent cytoplasmic vacuolation and inhibits the function of the lysosome in leukemia cells.Pharmaceutical science advances · 2024Article
- Molecular Regulation Mechanism of Microglial Autophagy in the Pathology of Alzheimer's Disease.Aging and disease · 2023Review
- Exercise-activated hepatic autophagy via the FN1-α5β1 integrin pathway drives metabolic benefits of exercise.Cell metabolism · 2023Article
- Individual Expression of Hepatitis A Virus 3C Protease Induces Ferroptosis in Human Cells In Vitro.International journal of molecular sciences · 2021Article
- Irisin and Autophagy: First Update.International journal of molecular sciences · 2020Review
- The Therapeutic and Pathogenic Role of Autophagy in Autoimmune Diseases.Frontiers in immunology · 2018Review
- Effects of chronic sugar consumption on lipid accumulation and autophagy in the skeletal muscle.European journal of nutrition · 2017Article
- PulmonaryFood & nutrition research · 2017Article
- Cytoplasmic vacuolization in cell death and survival.Oncotarget · 2016Review
- The effects and mechanism of estrogen on rats with Parkinson's disease in different age groups.American journal of translational research · 2016Article
- KIM-1-/TIM-1-mediated phagocytosis links ATG5-/ULK1-dependent clearance of apoptotic cells to antigen presentation.The EMBO journal · 2015Article
- Protease 3C of hepatitis A virus induces vacuolization of lysosomal/endosomal organelles and caspase-independent cell death.BMC cell biology · 2015Article
- Upregulation of cell surface estrogen receptor alpha is associated with the mitogen-activated protein kinase/extracellular signal-regulated kinase activity and promotes autophagy maturation.International journal of clinical and experimental pathology · 2015Article
- Autophagy inhibition by sustained overproduction of IL6 contributes to arsenic carcinogenesis.Cancer research · 2014Article
- Involvement of interleukin-1β in the autophagic process of microglia: relevance to Alzheimer's disease.Journal of neuroinflammation · 2013Article
Corrections and comments
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Increased autophagic vacuoles (AVs) occur in injured or degenerating neurons, under both developmental and pathological situations. Although an induced autophagy has been shown in inflammation response to cell factors, the underlying mechanism(s) remain(s) unknown. Here, we show that both cell factor IL-6 and environmental toxin MPP(+) promote the formation of vacuolation in SHSY5Y cells. By electron and immunofluorescent microscopy analyses, we showed that these structures are acid autolysosomes, containing cellular debris, and labeled by LC3 or LAMP1, markers of autophagosomes or lysosomes, respectively. Combining MPP(+) and IL-6 do not further increase vacuolation of SHSY5Y cells, and the vacuolation is less than that in the MPP(+)-treated group. MPP(+)-induced vacuolation results from significant increase in autophagy formation and delay in autophagy degradation, in relation to a decline of the lysosomal activity of arylsulfatase A. At molecular level, we show that this defect in autolysosomal maturation is independent of mammalian target of rapamycin and p38 inhibitions. Most importantly, we provide the first evidence that activation of ERK pathway is sufficient to commit cell to autophagic vacuolation. The sustained activation is required for MPP(+) to disrupt the autophagic pathway. IL-6 also induces a temporary and significant activation of ERK, but not sustained activation, and change sustained activation in MPP(+)-treated group into temporary activation. Taken together, these findings strongly support that IL-6 promotes the maturation of autophagosomes into functional autolysosomes by regulating ERK.
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