ArticleCytoskeleton (Hoboken, N.J.)2013
An optogenetic tool for the activation of endogenous diaphanous-related formins induces thickening of stress fibers without an increase in contractility.
Article in Cytoskeleton (Hoboken, N.J.), 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
29 citing papers in PubMed, 38 citations in OpenAlex.
- RhoA through its various effectors mediate mitochondrial fragmentation in polycystic kidney disease.iScience · 2026Article
- Article
- Turn-on protein switches for controlling actin binding in cells.Nature communications · 2024Article
- Turn-On Protein Switches for Controlling Actin Binding in Cells.bioRxiv : the preprint server for biology · 2023Article
- Light Activates Cdc42-Mediated Needle-Shaped Filopodia FormationCellular and molecular bioengineering · 2022Article
- Viscoelasticity, Like Forces, Plays a Role in Mechanotransduction.Frontiers in cell and developmental biology · 2022Review
- A guide to the optogenetic regulation of endogenous molecules.Nature methods · 2021Review
- A light way for nuclear cell biologists.Journal of biochemistry · 2021Review
- Engineering proteins for allosteric control by light or ligands.Nature protocols · 2019Article
- Review
- Control of microtubule dynamics using an optogenetic microtubule plus end-F-actin cross-linker.The Journal of cell biology · 2018Article
- Dissipation of contractile forces: the missing piece in cell mechanics.Molecular biology of the cell · 2017Article
- Optogenetic control of cellular forces and mechanotransduction.Nature communications · 2017Article
- Genetically Encoded Photoactuators and Photosensors for Characterization and Manipulation of Pluripotent Stem Cells.Theranostics · 2017Review
- Optogenetic Control of Protein Function: From Intracellular Processes to Tissue Morphogenesis.Trends in cell biology · 2016Review
- Formin-mediated actin polymerization at cell-cell junctions stabilizes E-cadherin and maintains monolayer integrity during wound repair.Molecular biology of the cell · 2016Article
- Optical Control of Peroxisomal Trafficking.ACS synthetic biology · 2016Article
- Correlating in Vitro and in Vivo Activities of Light-Inducible Dimers: A Cellular Optogenetics Guide.ACS synthetic biology · 2016Article
- Cell type-dependent mechanisms for formin-mediated assembly of filopodia.Molecular biology of the cell · 2015Article
- SMIFH2 has effects on Formins and p53 that perturb the cell cytoskeleton.Scientific reports · 2015Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors at 3 institutions in 2 countries.
Funding
Abstract
We have developed an optogenetic technique for the activation of diaphanous-related formins. Our approach is based on fusion of the light-oxygen-voltage 2 domain of Avena sativa Phototrophin1 to an isolated Diaphanous Autoregulatory Domain from mDia1. This "caged" diaphanous auto-regulatory domain was inactive in the dark but in the presence of blue light rapidly activated endogenous diaphanous-related formins. Using an F-actin reporter, we observed filopodia and lamellipodia formation as well as a steady increase in F-actin along existing stress fibers, starting within minutes of photo-activation. Interestingly, we did not observe the formation of new stress fibers. Remarkably, a 1.9-fold increase in F-actin was not paralleled by an increase in myosin II along stress fibers and the amount of tension generated by the fibers, as judged by focal adhesion size, appeared unchanged. Our results suggest a decoupling between F-actin accumulation and contractility in stress fibers and demonstrate the utility of photoactivatable diaphanous autoregulatory domain for the study of diaphanous-related formin function in cells.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.