ArticleJournal of industrial microbiology & biotechnology2013
Enhanced succinic acid production by Actinobacillus succinogenes after genome shuffling.
Article in Journal of industrial microbiology & biotechnology, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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Who cites it
12 citing papers in PubMed, 47 citations in OpenAlex.
- Transcriptional profiling reveals the effect of arginine on Actinobacillus succinogenes growth and fermentation.World journal of microbiology & biotechnology · 2025Article
- Immobilization of Actinobacillus succinogenes on nano- and micro-fiber membranes for efficient and robust production of succinic acid.Bioprocess and biosystems engineering · 2023Article
- Genome shuffling for improving the activity of alkaline pectinase in Bacillus subtilis FS105 and its molecular mechanism.World journal of microbiology & biotechnology · 2019Article
- Opportunities, challenges, and future perspectives of succinic acid production by Actinobacillus succinogenes.Applied microbiology and biotechnology · 2018Review
- Genome shuffling and high-throughput screening of Brevibacterium flavum MDV1 for enhanced L-valine production.World journal of microbiology & biotechnology · 2018Article
- Continuous succinic acid fermentation byBiotechnology for biofuels · 2018Article
- Metabolic Engineering of Actinobacillus succinogenes Provides Insights into Succinic Acid Biosynthesis.Applied and environmental microbiology · 2017Article
- Enhancing succinic acid biosynthesis in Escherichia coli by engineering its global transcription factor, catabolite repressor/activator (Cra).Scientific reports · 2016Article
- Microbial production of succinic acid using crude and purified glycerol from aBiotechnology reports (Amsterdam, Netherlands) · 2016Article
- Enhanced butanol production from cassava with Clostridium acetobutylicum by genome shuffling.World journal of microbiology & biotechnology · 2016Article
- Genome shuffling of the nonconventional yeast Pichia anomala for improved sugar alcohol production.Microbial cell factories · 2015Article
- Succinic acid production with Actinobacillus succinogenes: rate and yield analysis of chemostat and biofilm cultures.Microbial cell factories · 2014Article
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Succinic acid is an important platform chemical for synthesis of C4 compounds. We applied genome shuffling to improve fermentative production of succinic acid by A. succinogenes. Using a screening strategy composed of selection in fermentation broth, cultured in 96-deep-well plates, and condensed HPLC screening, a starting population of 11 mutants producing a higher succinic acid concentration was selected and subjected to recursive protoplasts fusion. After three rounds of genome shuffling, strain F3-II-3-F was obtained, producing succinic acid at 1.99 g/l/h with a yield of 95.6 g/l. The genome shuffled strain had about a 73 % improvement in succinic acid production compared to the parent strain after 48 h in fed-batch fermentation. The genomic variability of F3-II-3-F was confirmed by amplified fragment-length polymorphism. The activity levels of key enzymes involved in end-product formation from glucose and metabolic flux distribution during succinic acid production were compared between A. succinogenes CGMCC 1593 and F3-II-3-F. Increased activity of glucokinase, fructose-1,6-bisphosphate aldolase, PEP carboxykinase and fumarase, as well as decreased activity of pyruvate kinase, pyruvate formate-lyase, and acetate kinase explained the enhanced succinic acid production and decreased acetic acid formation. Metabolic flux analysis suggested that increased flux to NADH was the main reason for increased activity of the C4 pathway resulting in increased yields of succinic acid. The present work will be propitious to the development of a bio-succinic acid fermentation industry.
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