ArticleScientific reports2013
Highly rapid amplification-free and quantitative DNA imaging assay.
Article in Scientific reports, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
7 citing papers in PubMed.
- An open-source software analysis package for Microspheres with Ratiometric Barcode Lanthanide Encoding (MRBLEs).PloS one · 2019Article
- Multiplexed efficient on-chip sample preparation and sensitive amplification-free detection of Ebola virus.Biosensors & bioelectronics · 2017Article
- Single-Molecule Counting of Point Mutations by Transient DNA Binding.Scientific reports · 2017Article
- Lab on a single microbead: an ultrasensitive detection strategy enabling microRNA analysis at the single-molecule level.Chemical science · 2015Article
- Optofluidic analysis system for amplification-free, direct detection of Ebola infection.Scientific reports · 2015Article
- Droplet Digital Enzyme-Linked Oligonucleotide Hybridization Assay for Absolute RNA Quantification.Scientific reports · 2015Article
- Integration of programmable microfluidics and on-chip fluorescence detection for biosensing applications.Biomicrofluidics · 2014Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
10 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
There is an urgent need for rapid and highly sensitive detection of pathogen-derived DNA in a point-of-care (POC) device for diagnostics in hospitals and clinics. This device needs to work in a 'sample-in-result-out' mode with minimum number of steps so that it can be completely integrated into a cheap and simple instrument. We have developed a method that directly detects unamplified DNA, and demonstrate its sensitivity on realistically sized 5 kbp target DNA fragments of Micrococcus luteus in small sample volumes of 20 μL. The assay consists of capturing and accumulating of target DNA on magnetic beads with specific capture oligonucleotides, hybridization of complementary fluorescently labeled detection oligonucleotides, and fluorescence imaging on a miniaturized wide-field fluorescence microscope. Our simple method delivers results in less than 20 minutes with a limit of detection (LOD) of ~5 pM and a linear detection range spanning three orders of magnitude.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.