ArticleClinical and vaccine immunology : CVI2013
Impact of analytical variability on clinical interpretation of multiplex pneumococcal serology assays.
Article in Clinical and vaccine immunology : CVI, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed, 25 citations in OpenAlex.
- Response to Tetanus and Pneumococcal Vaccination Following Administration of Ixekizumab in Healthy Participants.BioDrugs : clinical immunotherapeutics, biopharmaceuticals and gene therapy · 2017Trial
- Quantitative Methods for Evaluating Antibody Responses to Pneumococcal Vaccines: A Scoping Review.Tropical medicine and infectious disease · 2025Review
- Reassessing Polysaccharide Responsiveness: Unveiling Limitations of Current Guidelines and Introducing the Polysaccharide Responsiveness Percentile Approach.Journal of clinical immunology · 2025Article
- Pneumococcal IgG Antibody Responses to 23vPPV in Healthy Controls Using an Automated ELISA.Journal of clinical immunology · 2022Article
- A Critical Review on the Standardization and Quality Assessment of Nonfunctional Laboratory Tests Frequently Used to Identify Inborn Errors of Immunity.Frontiers in immunology · 2021Review
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- Article
- Interlaboratory variability in multiplexed pneumococcal antibody testing.The Journal of allergy and clinical immunology · 2019Article
- Focusing on Good Responders to Pneumococcal Polysaccharide Vaccination in General Hospital Patients Suspected for Immunodeficiency. A Decision Tree Based on the 23-Valent Pneumococcal IgG Assay.Frontiers in immunology · 2019Article
- Measuring immune responses to pneumococcal vaccines.Journal of immunological methods · 2018Review
- Divergent humoral responses to 23-valent pneumococcal polysaccharide vaccine in critically-ill burn and neurosurgical patients.PloS one · 2018Observational
- Value of the Overall Pneumococcal Polysaccharide Response in the Diagnosis of Primary Humoral Immunodeficiencies.Frontiers in immunology · 2017Article
- Use and clinical interpretation of pneumococcal antibody measurements in the evaluation of humoral immune function.Clinical and vaccine immunology : CVI · 2015Review
- Multilaboratory assessment of threshold versus fold-change algorithms for minimizing analytical variability in multiplexed pneumococcal IgG measurements.Clinical and vaccine immunology : CVI · 2014Article
- Measurement of pneumococcal polysaccharide antibodies.Journal of clinical immunology · 2014Article
- Reference laboratory agreement on multianalyte pneumococcal antibody results: an absolute must!Clinical and vaccine immunology : CVI · 2013Article
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4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The response to pneumococcal vaccination can be used to assess a patient's humoral immune response to polysaccharide antigens. Multiplex assays measuring serotype-specific levels of pneumococcal antibodies are often used for this purpose, and clinical algorithms have been published to assist in the definition of an adequate immune response. We evaluated whether interlaboratory variability in multiplex pneumococcal serology assays would affect the clinical classification of the immune response. Specimens from 57 patients were analyzed at three reference laboratories with different multiplex assays to measure pneumococcal serology. Analytical correlation and clinical agreement in the classification of a patient's vaccination status by the three methods were compared. Although substantial variation in the quantitative antibody levels measured by different laboratories was seen, the qualitative classification of individual serologic results showed a high degree of agreement between labs and the ultimate classification of a patient as "protected" or "nonprotected" was the same for most patients. The majority of discordant classifications were driven by a systematic bias in results from one of the assays rather than by random error. These data suggest that the use of integrated assessments based on multiple serotypes can compensate for much of the analytical variability seen between laboratories. Knowledge of the analytical performance characteristics of a particular assay is most important when evaluating patients with results near clinical cut points.
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