ArticleTissue engineering. Part C, Methods2014
Hormonal regulation of epithelial organization in a three-dimensional breast tissue culture model.
Article in Tissue engineering. Part C, Methods, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed, 24 citations in OpenAlex.
- Modeling Mammary Organogenesis from Biological First Principles: A Systems Biology Approach.Methods in molecular biology (Clifton, N.J.) · 2024Article
- Matrix Composition Modulates Vitamin D3's Effects on 3D Collagen Fiber Organization by MCF10A Cells.Tissue engineering. Part A · 2021Article
- Effect of prolactin on normal and keratoconus human corneal stromal fibroblasts in vitro.PloS one · 2021Article
- Factors associated with obesity alter matrix remodeling in breast cancer tissues.Journal of biomedical optics · 2020Article
- Vitamin D3 constrains estrogen's effects and influences mammary epithelial organization in 3D cultures.Scientific reports · 2019Article
- Pathogenesis of Keratoconus: The intriguing therapeutic potential of Prolactin-inducible protein.Progress in retinal and eye research · 2018Review
- 3D organizational mapping of collagen fibers elucidates matrix remodeling in a hormone-sensitive 3D breast tissue model.Biomaterials · 2018Article
- Characterization of MCF-12A cell phenotype, response to estrogens, and growth in 3D.Cancer cell international · 2018Article
- Modeling mammary organogenesis from biological first principles: Cells and their physical constraints.Progress in biophysics and molecular biology · 2016Review
- A Hormone-responsive 3D Culture Model of the Human Mammary Gland Epithelium.Journal of visualized experiments : JoVE · 2016Article
- Gigantomastia and Macroprolactinemia Responding to Cabergoline Treatment: A Case Report and Minireview of the Literature.Case reports in endocrinology · 2016Article
- SAMA: A Method for 3D Morphological Analysis.PloS one · 2016Article
- Environmental endocrine disruptors: Effects on the human male reproductive system.Reviews in endocrine & metabolic disorders · 2015Review
- Rapid three-dimensional quantification of voxel-wise collagen fiber orientation.Biomedical optics express · 2015Article
- Dense collagen-I matrices enhance pro-tumorigenic estrogen-prolactin crosstalk in MCF-7 and T47D breast cancer cells.PloS one · 2015Article
- From single cells to tissues: interactions between the matrix and human breast cells in real time.PloS one · 2014Article
Corrections and comments
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Authors and funding
9 authors at 2 institutions in 2 countries.
Funding
Abstract
The establishment of hormone target breast cells in the 1970's resulted in suitable models for the study of hormone control of cell proliferation and gene expression using two-dimensional (2D) cultures. However, to study mammogenesis and breast tumor development in vitro, cells must be able to organize in three-dimensional (3D) structures like in the tissue. We now report the development of a hormone-sensitive 3D culture model for the study of mammogenesis and neoplastic development. Hormone-sensitive T47D breast cancer cells respond to estradiol in a dose-dependent manner by forming complex epithelial structures. Treatment with the synthetic progestagen promegestone, in the presence of estradiol, results in flat epithelial structures that display cytoplasmic projections, a phenomenon reported to precede side-branching. Additionally, as in the mammary gland, treatment with prolactin in the presence of estradiol induces budding structures. These changes in epithelial organization are accompanied by collagen remodeling. Collagen is the major acellular component of the breast stroma and an important player in tumor development and progression. Quantitative analysis of second harmonic generation of collagen fibers revealed that collagen density was more variable surrounding budding and irregularly shaped structures when compared to more regular structures; suggesting that fiber organization in the former is more anisotropic than in the latter. In sum, this new 3D model recapitulates morphogenetic events modulated by mammogenic hormones in the breast, and is suitable for the evaluation of therapeutic agents.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.