ArticlePloS one2013
Genomic approach to identify factors that drive the formation of three-dimensional structures by EA.hy926 endothelial cells.
Article in PloS one, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers.
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Who cites it
29 citing papers in PubMed, 54 citations in OpenAlex.
- Changes in morphology, gene expression and protein content in chondrocytes cultured on a random positioning machine.PloS one · 2013Trial
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- Omics Studies of Specialized Cells and Stem Cells under Microgravity Conditions.International journal of molecular sciences · 2024Review
- Structural and Molecular Changes of Human Chondrocytes Exposed to the Rotating Wall Vessel Bioreactor.Biomolecules · 2023Article
- Recent Advances in Breast Cancer Research.International journal of molecular sciences · 2023Article
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- Long-Term Simulation of Microgravity Induces Changes in Gene Expression in Breast Cancer Cells.International journal of molecular sciences · 2023Article
- Translation from Microgravity Research to Earth Application.International journal of molecular sciences · 2022Article
- Microgravity and Space Medicine 2.0.International journal of molecular sciences · 2022Article
- Space omics research in Europe: Contributions, geographical distribution and ESA member state funding schemes.iScience · 2022Review
- Meta-Analysis-Assisted Detection of Gravity-Sensitive Genes in Human Vascular Endothelial Cells.Frontiers in cell and developmental biology · 2021Article
- Alterations of Growth and Focal Adhesion Molecules in Human Breast Cancer Cells Exposed to the Random Positioning Machine.Frontiers in cell and developmental biology · 2021Article
- Dexamethasone Inhibits Spheroid Formation of Thyroid Cancer Cells Exposed to Simulated Microgravity.Cells · 2020Article
- Morphological and Molecular Changes in Juvenile Normal Human Fibroblasts Exposed to Simulated Microgravity.Scientific reports · 2019Article
- Microgravity Affects Thyroid Cancer Cells during the TEXUS-53 Mission Stronger than Hypergravity.International journal of molecular sciences · 2018Article
- Thyroid cancer cells in space during the TEXUS-53 sounding rocket mission - The THYROID Project.Scientific reports · 2018Article
- The role of NFκB in spheroid formation of human breast cancer cells cultured on the Random Positioning Machine.Scientific reports · 2018Article
- The Adverse Effect of Hypertension in the Treatment of Thyroid Cancer with Multi-Kinase Inhibitors.International journal of molecular sciences · 2017Review
- Identifications of novel mechanisms in breast cancer cells involving duct-like multicellular spheroid formation after exposure to the Random Positioning Machine.Scientific reports · 2016Article
Corrections and comments
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Authors and funding
8 authors at 4 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Understanding the mechanisms responsible for tube formation by endothelial cells (ECs) is of major interest and importance in medicine and tissue engineering. Endothelial cells of the human cell line EA.hy926 behave ambivalently when cultured on a random positioning machine (RPM) simulating microgravity. Some cells form tube-like three-dimensional (3D) aggregates, while other cells (AD) continue to grow adherently. Between the fifth and seventh day of culturing, the two types of cell growth achieve the greatest balance. We harvested ECs that grew either adherently or as 3D aggregates separately after five and seven days of incubation on the RPM, and applied gene array analysis and PCR techniques to investigate their gene expression profiles in comparison to ECs growing adherently under normal static 1 g laboratory conditions for equal periods of time. Using gene arrays, 1,625 differentially expressed genes were identified. A strong overrepresentation of transient expression differences was found in the five-day, RPM-treated samples, where the number of genes being differentially expressed in comparison to 1 g cells was highest as well as the degree of alteration regarding distinct genes. We found 27 genes whose levels of expression were changed at least 4-fold in RPM-treated cells as compared to 1 g controls. These genes code for signal transduction and angiogenic factors, cell adhesion, membrane transport proteins or enzymes involved in serine biosynthesis. Fifteen of them, with IL8 (interleukin 8) and VWF (von Willebrand factor) the most prominently affected, showed linkages to genes of another 20 proteins that are important in cell structure maintenance and angiogenesis and extended their network of interaction. Thus, the study reveals numerous genes, which mutually influence each other during initiation of 3D growth of endothelial cells.
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