ArticlePloS one2013
Mice lacking pten in osteoblasts have improved intramembranous and late endochondral fracture healing.
Article in PloS one, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed, 35 citations in OpenAlex.
- LINC00662 regulates osteogenic differentiation of BMSCs and inhibits fracture healing via miR-330-3p/PTEN axis.BMC musculoskeletal disorders · 2025Article
- Bone repair and key signalling pathways for cell-based bone regenerative therapy: A review.Journal of Taibah University Medical Sciences · 2023Review
- Protein tyrosine phosphatases in skeletal development and diseases.Bone research · 2022Review
- Review
- MiR-193a-3p Promotes Fracture Healing via Targeting PTEN Gene.Molecular biotechnology · 2021Article
- Critical implication of the PTEN/PI3K/AKT pathway during BMP2-induced heterotopic ossification.Molecular medicine reports · 2021Article
- The role of microRNAs in bone development.Bone · 2021Review
- Beneficial role of microRNA-328-3p in fracture healing by enhancing osteoblastic viability through the PTEN/PI3K/AKT pathway.Experimental and therapeutic medicine · 2020Article
- Pten deletion in Dmp1-expressing cells does not rescue the osteopenic effects of Wnt/β-catenin suppression.Journal of cellular physiology · 2020Article
- Piperlongumine Attenuates High Calcium/Phosphate-Induced Arterial Calcification by Preserving P53/PTEN Signaling.Frontiers in cardiovascular medicine · 2020Article
- A PDGFRβ-PI3K signaling axis mediates periosteal cell activation during fracture healing.PloS one · 2019Article
- [Effect of miR-21 down-regulated by HZhongguo xiu fu chong jian wai ke za zhi = Zhongguo xiufu chongjian waike zazhi = Chinese journal of reparative and reconstructive surgery · 2018Article
- PTEN Inhibition in Human Disease Therapy.Molecules (Basel, Switzerland) · 2018Review
- Desumoylase SENP6 maintains osteochondroprogenitor homeostasis by suppressing the p53 pathway.Nature communications · 2018Article
- Article
- Targeting of Mesenchymal Stromal Cells by Cre-Recombinase Transgenes Commonly Used to Target Osteoblast Lineage Cells.Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research · 2016Article
- Mice with a heterozygous Lrp6 deletion have impaired fracture healing.Bone research · 2016Article
- Fracture healing in mice lacking Pten in osteoblasts: a micro-computed tomography image-based analysis of the mechanical properties of the femur.Journal of biomechanics · 2015Article
- Role of Cbl-PI3K Interaction during Skeletal Remodeling in a Murine Model of Bone Repair.PloS one · 2015Article
- Phosphatase and Tensin Homologue: Novel Regulation by Developmental Signaling.Journal of signal transduction · 2015Review
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Authors and funding
11 authors at 4 institutions in 1 country.
Funding
Abstract
The failure of an osseous fracture to heal (development of a non-union) is a common and debilitating clinical problem. Mice lacking the tumor suppressor Pten in osteoblasts have dramatic and progressive increases in bone volume and density throughout life. Since fracture healing is a recapitulation of bone development, we investigated the process of fracture healing in mice lacking Pten in osteoblasts (Ocn-cre(tg/+;)Pten(flox/flox) ). Mid-diaphyseal femoral fractures induced in wild-type and Ocn-cre(tg/+;)Pten(flox/flox) mice were studied via micro-computed tomography (µCT) scans, biomechanical testing, histological and histomorphometric analysis, and protein expression analysis. Ocn-cre(tg/+;)Pten(flox/flox) mice had significantly stiffer and stronger intact bones relative to controls in all cohorts. They also had significantly stiffer healing bones at day 28 post-fracture (PF) and significantly stronger healing bones at days 14, 21, and 28 PF. At day 7 PF, the proximal and distal ends of the Pten mutant calluses were more ossified. By day 28 PF, Pten mutants had larger and more mineralized calluses. Pten mutants had improved intramembranous bone formation during healing originating from the periosteum. They also had improved endochondral bone formation later in the healing process, after mature osteoblasts are present in the callus. Our results indicate that the inhibition of Pten can improve fracture healing and that the local or short-term use of commercially available Pten-inhibiting agents may have clinical application for enhancing fracture healing.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.