ArticlePloS one2013
Engineering Pseudomonas protegens Pf-5 for nitrogen fixation and its application to improve plant growth under nitrogen-deficient conditions.
Article in PloS one, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. It carries an expression of concern. Cited by 39 papers.
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Who cites it
39 citing papers in PubMed, 99 citations in OpenAlex.
- Mechanisms of Pseudomonas spp. as plant growth promoting rhizobacteria: plant nutrition and biocontrol.Antonie van Leeuwenhoek · 2026Review
- Synthetic Biology of Plants and Microbes for Agriculture, Environment, and Future Applications.Chemical reviews · 2026Review
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- Effect ofLife (Basel, Switzerland) · 2025Article
- Next-generation biostimulants: molecular insights, digital integration, and regulatory frameworks for sustainable agriculture.Frontiers in plant science · 2025Review
- Genetic diversity, stress tolerance and phytobeneficial potential in rhizobacteria of Vachellia tortilis subsp. raddiana.Environmental microbiome · 2024Article
- Competitive fitness and stability of ammonium-excreting Azotobacter vinelandii strains in the soil.Applied microbiology and biotechnology · 2024Article
- Invisible Inhabitants of Plants and a Sustainable Planet: Diversity of Bacterial Endophytes and their Potential in Sustainable Agriculture.Indian journal of microbiology · 2024Review
- Scripting a new dialogue between diazotrophs and crops.Trends in microbiology · 2024Review
- Diurnal switches in diazotrophic lifestyle increase nitrogen contribution to cereals.Nature communications · 2023Article
- Engineering Nitrogenases for Synthetic Nitrogen Fixation: From Pathway Engineering to Directed Evolution.Biodesign research · 2023Review
- Pseudomonas cultivated from Andropogon gerardii rhizosphere show functional potential for promoting plant host growth and drought resilience.BMC genomics · 2022Article
- Maize Growth Promotion by Inoculation with an Engineered Ammonium-Excreting Strain of Nitrogen-FixingMicroorganisms · 2022Article
- Biological nitrogen fixation and prospects for ecological intensification in cereal-based cropping systems.Field crops research · 2022Review
- Enhanced growth of ginger plants by an eco- friendly nitrogen-fixing Pseudomonas protegens inoculant in glasshouse fields.Journal of the science of food and agriculture · 2022Article
- Pseudomonas putida and its close relatives: mixing and mastering the perfect tune for plants.Applied microbiology and biotechnology · 2022Review
- Genetic Determinants of Ammonium Excretion inApplied and environmental microbiology · 2022Article
- Effects of Phosphonate Herbicides on the Secretions of Plant-Beneficial Compounds by Two Plant Growth-Promoting Soil Bacteria: A Metabolomics Investigation.ACS environmental Au · 2022Article
- Diversity and adaptation properties of actinobacteria associated with Tunisian stone ruins.Frontiers in microbiology · 2022Article
- Effect of Individual, Simultaneous and Sequential Inoculation ofPlants (Basel, Switzerland) · 2021Article
Corrections and comments
- Erratum issued
- Expression of concern
Authors and funding
10 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Nitrogen is the second most critical factor for crop production after water. In this study, the beneficial rhizobacterium Pseudomonas protegens Pf-5 was genetically modified to fix nitrogen using the genes encoding the nitrogenase of Pseudomonas stutzeri A1501 via the X940 cosmid. Pf-5 X940 was able to grow in L medium without nitrogen, displayed high nitrogenase activity and released significant quantities of ammonium to the medium. Pf-5 X940 also showed constitutive expression and enzymatic activity of nitrogenase in ammonium medium or in nitrogen-free medium, suggesting a constitutive nitrogen fixation. Similar to Pseudomonas protegens Pf-5, Pseudomonas putida, Pseudomonas veronii and Pseudomonas taetrolens but not Pseudomonas balearica and Pseudomonas stutzeri transformed with cosmid X940 showed constitutive nitrogenase activity and high ammonium production, suggesting that this phenotype depends on the genome context and that this technology to obtain nitrogen-fixing bacteria is not restricted to Pf-5. Interestingly, inoculation of Arabidopsis, alfalfa, tall fescue and maize with Pf-5 X940 increased the ammonium concentration in soil and plant productivity under nitrogen-deficient conditions. In conclusion, these results open the way to the production of effective recombinant inoculants for nitrogen fixation on a wide range of crops.
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