ArticlePloS one2013
Actin microfilament mediates osteoblast Cbfa1 responsiveness to BMP2 under simulated microgravity.
Article in PloS one, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed, 44 citations in OpenAlex.
- The influence of simulated microgravity on MG-63 osteoblast-like cells cultured on polymeric scaffold.Biomaterials and biosystems · 2026Article
- Protection of primary cilia is an effective countermeasure against the impairment of osteoblast function induced by simulated microgravity.Journal of cellular and molecular medicine · 2023Article
- The actin cytoskeleton: Morphological changes in pre- and fully developed lung cancer.Biophysics reviews · 2022Review
- The effects of microgravity on bone structure and function.NPJ microgravity · 2022Review
- Bioinformatic analysis of the RNA expression patterns in microgravity-induced bone loss.Frontiers in genetics · 2022Article
- Simulating microgravity using a random positioning machine for inducing cellular responses to mechanotransduction in human osteoblasts.The Review of scientific instruments · 2021Article
- Mechanical Sensing Element PDLIM5 Promotes Osteogenesis of Human Fibroblasts by Affecting the Activity of Microfilaments.Biomolecules · 2021Article
- Low-intensity vibration restores nuclear YAP levels and acute YAP nuclear shuttling in mesenchymal stem cells subjected to simulated microgravity.NPJ microgravity · 2020Article
- Review
- Spaceflight-Induced Bone Tissue Changes that Affect Bone Quality and Increase Fracture Risk.Current osteoporosis reports · 2020Review
- Bioinspired Scaffold Action Under the Extreme Physiological Conditions of Simulated Space Flights: Osteogenesis Enhancing Under Microgravity.Frontiers in bioengineering and biotechnology · 2020Article
- Clinostat Rotation Affects Metabolite Transportation and Increases Organic Acid Production byApplied and environmental microbiology · 2019Article
- Gene Expression in Osteoblasts and Osteoclasts Under Microgravity Conditions: A Systematic Review.Current genomics · 2019Review
- Recovery of stem cell proliferation by low intensity vibration under simulated microgravity requires LINC complex.NPJ microgravity · 2019Article
- A Robust Actin Filaments Image Analysis Framework.PLoS computational biology · 2016Article
- Simulated microgravity inhibits osteogenic differentiation of mesenchymal stem cells via depolymerizing F-actin to impede TAZ nuclear translocation.Scientific reports · 2016Article
- Growing tissues in real and simulated microgravity: new methods for tissue engineering.Tissue engineering. Part B, Reviews · 2014Review
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Authors and funding
11 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Microgravity decreases osteoblastic activity, induces actin microfilament disruption and inhibits the responsiveness of osteoblast to cytokines, but the mechanisms remains enigmatic. The F-actin cytoskeleton has previously been implicated in manifold changes of cell shape, function and signaling observed under microgravity. Here we investigate the involvement of microfilament in mediating the effects of microgravity and BMP2 induction on Cbfa1 activity. For this purpose we constructed a fluorescent reporter cell line (OSE-MG63) of Cbfa1 activity by stably transfecting MG63 cells with a reporter consisting of six tandem copies of OSE2 and a minimal mOG2 promoter upstream of enhanced green fluorescent protein (EGFP). The fluorescence intensity of OSE-MG63 showed responsiveness to bone-related cytokines (IGF-I, vitamin D3 and BMP2) and presented an accordant tendency with alkaline phosphatase (ALP) activity. Using OSE-MG63 reporter fluorescence, we performed a semi-quantitative analysis of Cbfa1 activity after treatment with simulated microgravity, microfilament-disrupting agent (cytochalasin B, CB), microfilament-stabilizing agent (Jasplakinolide, JAS) or any combination thereof. In parallel, ALP activity, DNA binding activity of Cbfa1 to OSE2 (ChIP), F-actin structure (immunofluorescence) and EGFP mRNA expression (RT-qPCR) were analyzed. Simulated microgravity inhibited Cbfa1 activity, affected the responsiveness of Cbfa1 to cytokine BMP2, and caused a thinning and dispersed distribution of microfilament. Under normal gravity, CB significantly attenuated BMP2 induction to Cbfa1 activity as well as DNA binding activity of Cbfa1 to OSE2. The addition of JAS reversed the inhibitory effects of microgravity on the responsiveness of Cbfa1 to BMP2. Our study demonstrates that disrupting the microfilament organization by CB or simulated microgravity attenuates the responsiveness of Cbfa1 to BMP2. A stabilization of the microfilament organization by JAS reverses this inhibition. Taken together, these results suggest that actin microfilament participates in BMP2's induction to Cbfa1 activity and that their disruption might be an important contributor to microgravity's inhibition on BMP2's osteogenic induction.
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