ArticlePLoS genetics2013
Bck2 acts through the MADS box protein Mcm1 to activate cell-cycle-regulated genes in budding yeast.
Article in PLoS genetics, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed, 25 citations in OpenAlex.
- Single-cell imaging reveals a key role of Bck2 in yeast cell size adaptation to nutrient challenges.The Journal of cell biology · 2026Article
- Saccharomyces cerevisiae Mub1, a substrate adaptor of E3 ubiquitin ligase Ubr2, modulates sensitivity to cell wall stressors through multiple transcription factors.The FEBS journal · 2025Article
- The origin, evolution, and diversification of MADS-box transcription factors in green plants.Plant communications · 2025Article
- Mutations inJournal of fungi (Basel, Switzerland) · 2024Article
- Genomic analysis and mechanisms exploration of a stress tolerance and high-yield pullulan producing strain.Frontiers in genetics · 2024Article
- The microprotein Nrs1 rewires the G1/S transcriptional machinery during nitrogen limitation in budding yeast.PLoS biology · 2022Article
- Thidiazuron Promotes Leaf Abscission by Regulating the Crosstalk Complexities between Ethylene, Auxin, and Cytokinin in Cotton.International journal of molecular sciences · 2022Article
- Role of Calcium/Calcineurin Signalling in Regulating Intracellular Reactive Oxygen Species Homeostasis inGenes · 2021Article
- Msa1 and Msa2 Modulate G1-Specific Transcription to Promote G1 Arrest and the Transition to Quiescence in Budding Yeast.PLoS genetics · 2016Article
- The Biosynthetic Basis of Cell Size Control.Trends in cell biology · 2015Review
- Article
- STUbL-mediated degradation of the transcription factor MATα2 requires degradation elements that coincide with corepressor binding sites.Molecular biology of the cell · 2015Article
- Functional Analysis and Characterization of Differential Coexpression Networks.Scientific reports · 2015Article
- Multiple metabolic requirements for size homeostasis and initiation of division inMicrobial cell (Graz, Austria) · 2014Article
- Regulation of a transcription factor network by Cdk1 coordinates late cell cycle gene expression.The EMBO journal · 2014Article
- PP2ARts1 is a master regulator of pathways that control cell size.The Journal of cell biology · 2014Article
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
The Bck2 protein is a potent genetic regulator of cell-cycle-dependent gene expression in budding yeast. To date, most experiments have focused on assessing a potential role for Bck2 in activation of the G1/S-specific transcription factors SBF (Swi4, Swi6) and MBF (Mbp1, Swi6), yet the mechanism of gene activation by Bck2 has remained obscure. We performed a yeast two-hybrid screen using a truncated version of Bck2 and discovered six novel Bck2-binding partners including Mcm1, an essential protein that binds to and activates M/G1 promoters through Early Cell cycle Box (ECB) elements as well as to G2/M promoters. At M/G1 promoters Mcm1 is inhibited by association with two repressors, Yox1 or Yhp1, and gene activation ensues once repression is relieved by an unknown activating signal. Here, we show that Bck2 interacts physically with Mcm1 to activate genes during G1 phase. We used chromatin immunoprecipitation (ChIP) experiments to show that Bck2 localizes to the promoters of M/G1-specific genes, in a manner dependent on functional ECB elements, as well as to the promoters of G1/S and G2/M genes. The Bck2-Mcm1 interaction requires valine 69 on Mcm1, a residue known to be required for interaction with Yox1. Overexpression of BCK2 decreases Yox1 localization to the early G1-specific CLN3 promoter and rescues the lethality caused by overexpression of YOX1. Our data suggest that Yox1 and Bck2 may compete for access to the Mcm1-ECB scaffold to ensure appropriate activation of the initial suite of genes required for cell cycle commitment.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.