ArticleMolecular & cellular proteomics : MCP2013
Mapping the extracellular and membrane proteome associated with the vasculature and the stroma in the embryo.
Article in Molecular & cellular proteomics : MCP, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers.
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Who cites it
7 citing papers in PubMed, 25 citations in OpenAlex.
- Evolution of the Chick Embryo Chorioallantoic Membrane Proteome during Early Development.ACS omega · 2026Article
- Identification of Potential Abnormal Methylation-Modified Genes in Coronary Artery Ectasia.International journal of genomics · 2023Article
- Proteome of the Luminal Surface of the Blood-Brain Barrier.Proteomes · 2021Review
- 3D Mapping Reveals a Complex and Transient Interstitial Matrix During Murine Kidney Development.Journal of the American Society of Nephrology : JASN · 2021Article
- Review
- Consensus guidelines for the use and interpretation of angiogenesis assays.Angiogenesis · 2018Review
- The invasive proteome of glioblastoma revealed by laser-capture microdissection.Neuro-oncology advancesArticle
Corrections and comments
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Authors and funding
10 authors at 7 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
In order to map the extracellular or membrane proteome associated with the vasculature and the stroma in an embryonic organism in vivo, we developed a biotinylation technique for chicken embryo and combined it with mass spectrometry and bioinformatic analysis. We also applied this procedure to implanted tumors growing on the chorioallantoic membrane or after the induction of granulation tissue. Membrane and extracellular matrix proteins were the most abundant components identified. Relative quantitative analysis revealed differential protein expression patterns in several tissues. Through a bioinformatic approach, we determined endothelial cell protein expression signatures, which allowed us to identify several proteins not yet reported to be associated with endothelial cells or the vasculature. This is the first study reported so far that applies in vivo biotinylation, in combination with robust label-free quantitative proteomics approaches and bioinformatic analysis, to an embryonic organism. It also provides the first description of the vascular and matrix proteome of the embryo that might constitute the starting point for further developments.
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Registered trials
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