ArticleThe Plant cell2013
Golgi traffic and integrity depend on N-myristoyl transferase-1 in Arabidopsis.
Article in The Plant cell, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed, 39 citations in OpenAlex.
- Identification of candidate genes and development of KASP markers for soybean pod-related traits using GWAS.Frontiers in plant science · 2025Article
- ER-associated VAP27-1 and VAP27-3 proteins functionally link the lipid-binding ORP2A at the ER-chloroplast contact sites.Nature communications · 2024Article
- Functional Characterization of the Cystine-Rich-Receptor-like Kinases (International journal of molecular sciences · 2022Article
- Dual Role of ACBD6 in the Acylation Remodeling of Lipids and Proteins.Biomolecules · 2022Article
- ESCRT components ISTL1 andLIP5 are required for tapetal function and pollen viability.The Plant cell · 2021Article
- Homeostasis of branched-chain amino acids is critical for the activity of TOR signaling ineLife · 2019Article
- AtCRK5 Protein Kinase Exhibits a Regulatory Role in Hypocotyl Hook Development during Skotomorphogenesis.International journal of molecular sciences · 2019Article
- Unique features in the intracellular transport of typhoid toxin revealed by a genome-wide screen.PLoS pathogens · 2019Article
- TGNap1 is required for microtubule-dependent homeostasis of a subpopulation of the plant trans-Golgi network.Nature communications · 2018Article
- Investigation of Novel Regulation of N-myristoyltransferase by Mammalian Target of Rapamycin in Breast Cancer Cells.Scientific reports · 2018Article
- Salicylic acid-independent role of NPR1 is required for protection from proteotoxic stress in the plant endoplasmic reticulum.Proceedings of the National Academy of Sciences of the United States of America · 2018Article
- Functional Analysis of theInternational journal of molecular sciences · 2018Article
- Targeted Profiling ofThe Plant cell · 2018Article
- Variation in Membrane Trafficking Linked to SNARE AtSYP51 Interaction With Aquaporin NIP1;1.Frontiers in plant science · 2018Article
- Advances in Plant ER Architecture and Dynamics.Plant physiology · 2018Review
- HLB1 Is a Tetratricopeptide Repeat Domain-Containing Protein That Operates at the Intersection of the Exocytic and Endocytic Pathways at the TGN/EE in Arabidopsis.The Plant cell · 2016Article
- Detecting N-myristoylation and S-acylation of host and pathogen proteins in plants using click chemistry.Plant methods · 2016Article
- N-terminal modifications contribute to flowering time and immune response regulations.Plant signaling & behavior · 2015Article
- The role of lipid post-translational modification in plant developmental processes.Frontiers in plant science · 2014Review
- Endomembrane and Golgi traffic in plant cells.Methods in cell biology · 2013Article
Corrections and comments
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Authors and funding
7 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
N-myristoylation is a crucial irreversible eukaryotic lipid modification allowing a key subset of proteins to be targeted at the periphery of specific membrane compartments. Eukaryotes have conserved N-myristoylation enzymes, involving one or two N-myristoyltransferases (NMT1 and NMT2), among which NMT1 is the major enzyme. In the postembryonic developmental stages, defects in NMT1 lead to aberrant cell polarity, flower differentiation, fruit maturation, and innate immunity; however, no specific NMT1 target responsible for such deficiencies has hitherto been identified. Using a confocal microscopy forward genetics screen for the identification of Arabidopsis thaliana secretory mutants, we isolated STINGY, a recessive mutant with defective Golgi traffic and integrity. We mapped STINGY to a substitution at position 160 of Arabidopsis NMT1 (NMT1A160T). In vitro kinetic studies with purified NMT1A160T enzyme revealed a significant reduction in its activity due to a remarkable decrease in affinity for both myristoyl-CoA and peptide substrates. We show here that this recessive mutation is responsible for the alteration of Golgi traffic and integrity by predominantly affecting the Golgi membrane/cytosol partitioning of ADP-ribosylation factor proteins. Our results provide important functional insight into N-myristoylation in plants by ascribing postembryonic functions of Arabidopsis NMT1 that involve regulation of the functional and morphological integrity of the plant endomembranes.
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