ArticleThe Plant cell2013
Inactivation of plasma membrane-localized CDPK-RELATED KINASE5 decelerates PIN2 exocytosis and root gravitropic response in Arabidopsis.
Article in The Plant cell, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 46 papers.
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Who cites it
46 citing papers in PubMed, 101 citations in OpenAlex.
- Calmodulin-dependent protein kinase-related kinase CRK5 contributes to the regulation of the PIN3 cellular localization domain upon plant weight increase.Plant & cell physiology · 2026Article
- Genus-Wide Pan-Genome Analysis ofPlants (Basel, Switzerland) · 2025Article
- Genome-Wide Investigation ofInternational journal of molecular sciences · 2025Article
- Genome-wide analysis of CBL and CIPK gene families in bermudagrass reveals the CdCIPK29-A1 as a stem growth angle regulator.Plant cell reports · 2025Article
- Mutation in Arabidopsis mitochondrial Pentatricopeptide repeat 40 gene affects tolerance to water deficit.Planta · 2024Article
- Characterization of the PIN Auxin Efflux Carrier Gene Family and Its Expression during Zygotic Embryogenesis inPlants (Basel, Switzerland) · 2023Article
- Dynamic GOLVEN-ROOT GROWTH FACTOR 1 INSENSITIVE signaling in the root cap mediates root gravitropism.Plant physiology · 2023Article
- Calcium-dependent protein kinase CDPK16 phosphorylates serine-856 of glutamate receptor-like GLR3.6 protein leading to salt-responsive root growth in ArabidopsisFrontiers in plant science · 2023Article
- Functional Characterization of the Cystine-Rich-Receptor-like Kinases (International journal of molecular sciences · 2022Article
- Plants in Microgravity: Molecular and Technological Perspectives.International journal of molecular sciences · 2022Review
- Auxin Transporters-A Biochemical View.Cold Spring Harbor perspectives in biology · 2022Review
- Subcellular trafficking and post-translational modification regulate PIN polarity in plants.Frontiers in plant science · 2022Review
- Characterization of a Novel Creeping Tartary Buckwheat (Frontiers in plant science · 2022Article
- Adaptive divergence in shoot gravitropism creates hybrid sterility in an Australian wildflower.Proceedings of the National Academy of Sciences of the United States of America · 2021Article
- Molecular Evolution of Calcium Signaling and Transport in Plant Adaptation to Abiotic Stress.International journal of molecular sciences · 2021Review
- Calcium-dependent protein kinase 29 modulates PIN-FORMED polarity and Arabidopsis development via its own phosphorylation code.The Plant cell · 2021Article
- The AtCRK5 Protein Kinase Is Required to Maintain the ROS NO Balance Affecting the PIN2-Mediated Root Gravitropic Response in Arabidopsis.International journal of molecular sciences · 2021Article
- Analysis of Graviresponse and Biological Effects of Vertical and Horizontal Clinorotation inPlants (Basel, Switzerland) · 2021Article
- Auxin and Root Gravitropism: Addressing Basic Cellular Processes by Exploiting a Defined Growth Response.International journal of molecular sciences · 2021Review
- Genome-Wide Identification and Characterization ofInternational journal of molecular sciences · 2021Article
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Authors and funding
13 authors at 4 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
CRK5 is a member of the Arabidopsis thaliana Ca(2+)/calmodulin-dependent kinase-related kinase family. Here, we show that inactivation of CRK5 inhibits primary root elongation and delays gravitropic bending of shoots and roots. Reduced activity of the auxin-induced DR5-green fluorescent protein reporter suggests that auxin is depleted from crk5 root tips. However, no tip collapse is observed and the transcription of genes for auxin biosynthesis, AUXIN TRANSPORTER/AUXIN TRANSPORTER-LIKE PROTEIN (AUX/LAX) auxin influx, and PIN-FORMED (PIN) efflux carriers is unaffected by the crk5 mutation. Whereas AUX1, PIN1, PIN3, PIN4, and PIN7 display normal localization, PIN2 is depleted from apical membranes of epidermal cells and shows basal to apical relocalization in the cortex of the crk5 root transition zone. This, together with an increase in the number of crk5 lateral root primordia, suggests facilitated auxin efflux through the cortex toward the elongation zone. CRK5 is a plasma membrane-associated kinase that forms U-shaped patterns facing outer lateral walls of epidermis and cortex cells. Brefeldin inhibition of exocytosis stimulates CRK5 internalization into brefeldin bodies. CRK5 phosphorylates the hydrophilic loop of PIN2 in vitro, and PIN2 shows accelerated accumulation in brefeldin bodies in the crk5 mutant. Delayed gravitropic response of the crk5 mutant thus likely reflects defective phosphorylation of PIN2 and deceleration of its brefeldin-sensitive membrane recycling.
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