ArticleJournal of the American Chemical Society2013
Mechanistic insights into the LsrK kinase required for autoinducer-2 quorum sensing activation.
Article in Journal of the American Chemical Society, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed, 28 citations in OpenAlex.
- Autoinducer 2 as a universal language in microbial consortia: decoding molecular mechanisms, ecological impacts, and application.Gut microbes · 2026Review
- New LsrK Ligands as AI-2 Quorum Sensing Interfering Compounds against Biofilm Formation.Journal of medicinal chemistry · 2024Article
- Kaempferol restores the susceptibility of ESBLsFrontiers in microbiology · 2024Article
- Virtual screening-based discovery of AI-2 quorum sensing inhibitors that interact with an allosteric hydrophobic site of LsrK and their functional evaluation.Frontiers in chemistry · 2023Article
- Regulatory Mechanisms between Quorum Sensing and Virulence inMicroorganisms · 2022Review
- Biofilm formation and inhibition mediated by bacterial quorum sensing.Applied microbiology and biotechnology · 2022Review
- The role of bacterial signaling networks in antibiotics response and resistance regulation.Marine life science & technology · 2022Review
- Structural Characterization of LsrK as a Quorum Sensing Target and a Comparison between X-ray and Homology Models.Journal of chemical information and modeling · 2021Article
- Tackling Antimicrobial Resistance with Small Molecules Targeting LsrK: Challenges and Opportunities.Journal of medicinal chemistry · 2020Article
- Quorum-Sensing Regulation of Antimicrobial Resistance in Bacteria.Microorganisms · 2020Review
- Regulatory Mechanisms of the LuxS/AI-2 System and Bacterial Resistance.Antimicrobial agents and chemotherapy · 2019Review
- A Versatile Strategy for the Synthesis of 4,5-Dihydroxy-2,3-Pentanedione (DPD) and Related Compounds as Potential Modulators of Bacterial Quorum Sensing.Molecules (Basel, Switzerland) · 2018Article
- Evidence of link between quorum sensing and sugar metabolism inScience advances · 2018Article
- LsrF, a coenzyme A-dependent thiolase, catalyzes the terminal step in processing the quorum sensing signal autoinducer-2.Proceedings of the National Academy of Sciences of the United States of America · 2014Article
- Small molecule inhibitors of AI-2 signaling in bacteria: state-of-the-art and future perspectives for anti-quorum sensing agents.International journal of molecular sciences · 2013Article
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 2 countries.
Funding
Abstract
In enteric bacteria, the kinase LsrK catalyzes the phosphorylation of the C5-hydroxyl group in the linear form of 4,5-dihydroxy-2,3-pentanedione (DPD), the precursor of the type II bacterial quorum sensing molecule (AI-2). This phosphorylation is required for AI-2 sequestration in the cytoplasm and subsequent derepression of AI-2-related genes necessary for quorum development. While LsrK is a critical enzyme within the DPD quorum sensing relay system, kinetic details of this kinase have yet to be reported. A continuous UV-vis spectrophotometric assay was developed that allowed steady-state kinetic analysis of LsrK to be undertaken with the substrates ATP and DPD. The data was most consistent with a rapid equilibrium ordered mechanism with ATP binding first: kcat (7.4 ± 0.6 s(-1)), Km,ATP (150 ± 30 μM) and Km(app),DPD (1.0 ± 0.2 mM). The assay also allowed a DPD substrate profile to be conducted, which provided an unexpected biochemical disconnect between the previous agonist/antagonist cell-based reporter assay and the LsrK assay presented herein. Together these findings raise the importance of LsrK and lay the foundation not only for further understanding of this enzyme and its critical biological role but also for the rational design of regulatory molecules targeting AI-2 quorum sensing in pathogenic bacteria.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.