ArticleAnalytical chemistry2013
Western blotting using microchip electrophoresis interfaced to a protein capture membrane.
Article in Analytical chemistry, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
16 citing papers in PubMed, 44 citations in OpenAlex.
- Comprehensive Optimization of Western Blotting.Gels (Basel, Switzerland) · 2023Article
- Capillary electrophoresis Western blot using inkjet transfer to membrane.Journal of chromatography. A · 2022Article
- The Intriguing Landscape of Single-Cell Protein Analysis.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2022Review
- Fast Immunoassay for Microfluidic Western Blotting by Direct Deposition of Reagents onto Capture Membrane.Analytical methods : advancing methods and applications · 2020Article
- Review
- Design and microfabrication of a miniature fiber optic probe with integrated lenses and mirrors for Raman and fluorescence measurements.Analytical and bioanalytical chemistry · 2017Article
- Recent Advances in Microscale Western Blotting.Analytical methods : advancing methods and applications · 2016Article
- Multiplexed Western Blotting Using Microchip Electrophoresis.Analytical chemistry · 2016Article
- Hydrogel Pore-Size Modulation for Enhanced Single-Cell Western Blotting.Advanced materials (Deerfield Beach, Fla.) · 2016Article
- Rapid, Multiplexed Phosphoprotein Profiling Using Silicon Photonic Sensor Arrays.ACS central science · 2015Article
- Aptamer-based microchip electrophoresis assays for amplification detection of carcinoembryonic antigen.Clinica chimica acta; international journal of clinical chemistry · 2015Article
- Applications of microfluidics and microchip electrophoresis for potential clinical biomarker analysis.Analytical and bioanalytical chemistry · 2015Review
- Microfluidic Western blotting of low-molecular-mass proteins.Analytical chemistry · 2014Article
- Recent advances in the analysis of therapeutic proteins by capillary and microchip electrophoresis.Analytical methods : advancing methods and applications · 2014Article
- Microfluidic Valves Made From Polymerized Polyethylene Glycol Diacrylate.Sensors and actuators. B, Chemical · 2014Article
- Microfluidic chip-based technologies: emerging platforms for cancer diagnosis.BMC biotechnology · 2013Review
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
Western blotting is a commonly used assay for proteins. Despite the utility of the method, it is also characterized by long analysis times, manual operation, and lack of established miniaturized counterpart. We report a new way to Western blot that addresses these limitations. In the method, sodium dodecyl sulfate (SDS)-protein complexes are separated by sieving electrophoresis in a microfluidic device or chip. The chip is interfaced to a moving membrane so that proteins are captured in discrete zones as they migrate from the chip. Separations of SDS-protein complexes in the molecular weight range of 11-155 kDa were completed in 2 min with 4 × 10(4) theoretical plates at 460 V/cm. Migration time and peak area relative standard deviations were 3-6% and 0.2%, respectively. Detection limit for actin was 0.7 nM. Assays for actin, AMP-kinase, carbonic anhydrase, and lysozyme are shown to demonstrate versatility of the method. Total analysis time including immunoassay was 22-32 min for a single sample. Because processing membrane for immunoassay is the slow step of the assay, sequential injections from different reservoirs on the chip and capture in different tracks on the same membrane allow increased throughput. As a demonstration, 9 injections were collected on one membrane and analyzed in 43 min (~5 min/sample). Further improvements in throughput are possible with more reservoirs or parallel channels.
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What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.