ArticleZebrafish2013
Deriving cell lines from zebrafish embryos and tumors.
Article in Zebrafish, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
14 citing papers in PubMed.
- Protocol for deriving proliferating primary fibroblast cultures from Malabar red snapper larvae for cytogenetic analysis.STAR protocols · 2026Article
- Emerging Frontiers in Zebrafish Embryonic and Adult-Derived Cell Lines.International journal of molecular sciences · 2025Review
- Pluripotent cells derived from 50% epiboly zebrafish embryos.In vitro cellular & developmental biology. Animal · 2025Article
- Zebrafish-A Suitable Model for Rapid Translation of Effective Therapies for Pediatric Cancers.Cancers · 2024Review
- Advances in development of long-term embryonic stem cell-like cultures from a marine fish,Current research in food science · 2024Article
- Bioevaluation of magnetic mesoporous silica rods: cytotoxicity, cell uptake and biodistribution in zebrafish and rodents.RSC advances · 2022Article
- Generation of Humanized Zebrafish Models for the In Vivo Assessment of Antisense Oligonucleotide-Based Splice Modulation Therapies.Methods in molecular biology (Clifton, N.J.) · 2022Article
- The Last Half Century of Fish Explant and Organ Culture.Zebrafish · 2021Article
- Ganglioneuromas are driven by activated AKT and can be therapeutically targeted with mTOR inhibitors.The Journal of experimental medicine · 2020Article
- Meltome atlas-thermal proteome stability across the tree of life.Nature methods · 2020Article
- Versican is crucial for the initiation of cardiovascular lumen development in medaka (Oryzias latipes).Scientific reports · 2019Article
- Common midwife toad ranaviruses replicate first in the oral cavity of smooth newts (Lissotriton vulgaris) and show distinct strain-associated pathogenicity.Scientific reports · 2019Article
- Culture and Transfection of Zebrafish Primary Cells.Journal of visualized experiments : JoVE · 2018Article
- Ranavirus genotypes in the Netherlands and their potential association with virulence in water frogs (Pelophylax spp.).Emerging microbes & infections · 2018Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Over the last two decades the zebrafish has emerged as a powerful model organism in science. The experimental accessibility, the broad range of zebrafish mutants, and the highly conserved genetic and biochemical pathways between zebrafish and mammals lifted zebrafish to become one of the most attractive vertebrate models to study gene function and to model human diseases. Zebrafish cell lines are highly attractive to investigate cell biology and zebrafish cell lines complement the experimental tools that are available already. We established a straightforward method to culture cells from a single zebrafish embryo or a single tumor. Here we describe the generation of fibroblast-like cell lines from wild-type and ptenb(-/-) embryos and an endothelial-like cell line from a tumor of an adult ptena(+/-)ptenb(-/-) zebrafish. This protocol can easily be adapted to establish stable cell lines from any mutant or transgenic zebrafish line and the average time to obtain a pro-stable cell line is 3-5 months.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.