ArticlePloS one2013
The sRNA RyhB regulates the synthesis of the Escherichia coli methionine sulfoxide reductase MsrB but not MsrA.
Article in PloS one, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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Who cites it
12 citing papers in PubMed, 23 citations in OpenAlex.
- Exploring the targetome of IsrR, an iron-regulated sRNA controlling the synthesis of iron-containing proteins inFrontiers in microbiology · 2024Article
- Methionine oxidation under anaerobic conditions in Escherichia coli.Molecular microbiology · 2022Article
- Prediction of Bacterial sRNAs Using Sequence-Derived Features and Machine Learning.Bioinformatics and biology insights · 2022Article
- Article
- The Role of Methionine Sulfoxide Reductases in Oxidative Stress Tolerance and Virulence ofAntioxidants (Basel, Switzerland) · 2018Review
- Contrasting silencing mechanisms of the same target mRNA by two regulatory RNAs in Escherichia coli.Nucleic acids research · 2018Article
- Bacterial Iron Homeostasis Regulation by sRNAs.Microbiology spectrum · 2018Review
- The Small RNA ErsA ofFrontiers in microbiology · 2018Article
- IntaRNA 2.0: enhanced and customizable prediction of RNA-RNA interactions.Nucleic acids research · 2017Article
- sRNA roles in regulating transcriptional regulators: Lrp and SoxS regulation by sRNAs.Nucleic acids research · 2016Article
- Regulation of Expression of Oxacillin-Inducible Methionine Sulfoxide Reductases in Staphylococcus aureus.International journal of microbiology · 2015Article
- Metal limitation and toxicity at the interface between host and pathogen.FEMS microbiology reviews · 2014Review
Corrections and comments
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Authors and funding
8 authors at 4 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Controlling iron homeostasis is crucial for all aerobically grown living cells that are exposed to oxidative damage by reactive oxygen species (ROS), as free iron increases the production of ROS. Methionine sulfoxide reductases (Msr) are key enzymes in repairing ROS-mediated damage to proteins, as they reduce oxidized methionine (MetSO) residues to methionine. E. coli synthesizes two Msr, A and B, which exhibit substrate diastereospecificity. The bacterial iron-responsive small RNA (sRNA) RyhB controls iron metabolism by modulating intracellular iron usage. We show in this paper that RyhB is a direct regulator of the msrB gene that encodes the MsrB enzyme. RyhB down-regulates msrB transcripts along with Hfq and RNaseE proteins since mutations in the ryhB, fur, hfq, or RNaseE-encoded genes resulted in iron-insensitive expression of msrB. Our results show that RyhB binds to two sequences within the short 5'UTR of msrB mRNA as identified by reverse transcriptase and RNase and lead (II) protection assays. Toeprinting analysis shows that RyhB pairing to msrB mRNA prevents efficient ribosome binding and thereby inhibits translation initiation. In vivo site directed-mutagenesis experiments in the msrB 5'UTR region indicate that both RyhB-pairing sites are required to decrease msrB expression. Thus, this study suggests a novel mechanism of translational regulation where a same sRNA can basepair to two different locations within the same mRNA species. In contrast, expression of msrA is not influenced by changes in iron levels.
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