ArticlePloS one2013
Multiplex profiling of cellular invasion in 3D cell culture models.
Article in PloS one, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
17 citing papers in PubMed, 37 citations in OpenAlex.
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- Phenotypic drug screening in a human fibrosis model identified a novel class of antifibrotic therapeutics.Science advances · 2021Article
- Extracellular matrix remodelling in COPD.European respiratory review : an official journal of the European Respiratory Society · 2020Review
- TGFβ-induced cytoskeletal remodeling mediates elevation of cell stiffness and invasiveness in NSCLC.Scientific reports · 2019Article
- Expression ratio of the TGFβ-inducible gene MYO10 is prognostic for overall survival of squamous cell lung cancer patients and predicts chemotherapy response.Scientific reports · 2018Article
- Cancer cell specific inhibition of Wnt/β-catenin signaling by forced intracellular acidification.Cell discovery · 2018Article
- Specific recruitment of circulating angiogenic cells using biomaterials as filters.Acta biomaterialia · 2017Article
- 3D pulmospheres serve as a personalized and predictive multicellular model for assessment of antifibrotic drugs.JCI insight · 2017Article
- Fibrogenic Lung Injury Induces Non-Cell-Autonomous Fibroblast Invasion.American journal of respiratory cell and molecular biology · 2016Article
- Multidimensional immunolabeling and 4D time-lapse imaging of vital ex vivo lung tissue.American journal of physiology. Lung cellular and molecular physiology · 2015Article
- Validated prediction of pro-invasive growth factors using a transcriptome-wide invasion signature derived from a complex 3D invasion assay.Scientific reports · 2015Article
- Quantification of breast cancer cell invasiveness using a three-dimensional (3D) model.Journal of visualized experiments : JoVE · 2014Article
- Developments in preclinical cancer imaging: innovating the discovery of therapeutics.Nature reviews. Cancer · 2014Review
- The need for complex 3D culture models to unravel novel pathways and identify accurate biomarkers in breast cancer.Advanced drug delivery reviews · 2014Review
- Podosomes in space: macrophage migration and matrix degradation in 2D and 3D settings.Cell adhesion & migration · 2014Review
Corrections and comments
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Authors and funding
5 authors at 4 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
To-date, most invasion or migration assays use a modified Boyden chamber-like design to assess migration as single-cell or scratch assays on coated or uncoated planar plastic surfaces. Here, we describe a 96-well microplate-based, high-content, three-dimensional cell culture assay capable of assessing invasion dynamics and molecular signatures thereof. On applying our invasion assay, we were able to demonstrate significant effects on the invasion capacity of fibroblast cell lines, as well as primary lung fibroblasts. Administration of epidermal growth factor resulted in a substantial increase of cellular invasion, thus making this technique suitable for high-throughput pharmacological screening of novel compounds regulating invasive and migratory pathways of primary cells. Our assay also correlates cellular invasiveness to molecular events. Thus, we argue of having developed a powerful and versatile toolbox for an extensive profiling of invasive cells in a 96-well format. This will have a major impact on research in disease areas like fibrosis, metastatic cancers, or chronic inflammatory states.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.