ArticlePloS one2013
In situ tip-recordings found no evidence for an Orco-based ionotropic mechanism of pheromone-transduction in Manduca sexta.
Article in PloS one, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed, 41 citations in OpenAlex.
- Pheromone-Binding Proteins in Pest Control: From Molecular Insights to Real-World Applications.Journal of agricultural and food chemistry · 2025Review
- Hawkmoth Pheromone Transduction Involves G-Protein-Dependent Phospholipase Cβ Signaling.eNeuro · 2025Article
- Modulation of the NO-cGMP pathway has no effect on olfactory responses in theFrontiers in cellular neuroscience · 2023Article
- Altered functional properties of the codling moth Orco mutagenized in the intracellular loop-3.Scientific reports · 2021Article
- Mutagenesis of odorant coreceptorProceedings of the National Academy of Sciences of the United States of America · 2019Article
- Access to the odor world: olfactory receptors and their role for signal transduction in insects.Cellular and molecular life sciences : CMLS · 2018Review
- Tuning Insect Odorant Receptors.Frontiers in cellular neuroscience · 2018Article
- The Diacylglycerol Analogs OAG and DOG Differentially Affect Primary Events of Pheromone Transduction in the HawkmothFrontiers in cellular neuroscience · 2018Article
- The Two Main Olfactory Receptor Families inFrontiers in cellular neuroscience · 2018Review
- Insect Pheromone Receptors - Key Elements in Sensing Intraspecific Chemical Signals.Frontiers in cellular neuroscience · 2018Review
- Key Odorants Regulate Food Attraction inFrontiers in behavioral neuroscience · 2017Article
- Morphological and Transcriptomic Analysis of a Beetle Chemosensory System Reveals a Gnathal Olfactory Center.BMC biology · 2016Article
- Article
- Octopamine regulates antennal sensory neurons via daytime-dependent changes in cAMP and IP3 levels in the hawkmoth Manduca sexta.PloS one · 2015Article
- Peripheral olfactory signaling in insects.Current opinion in insect science · 2014Article
- An inhibitor of Na(+)/Ca(2+) exchange blocks activation of insect olfactory receptors.Biochemical and biophysical research communications · 2014Article
- Odorant receptor-mediated sperm activation in disease vector mosquitoes.Proceedings of the National Academy of Sciences of the United States of America · 2014Article
- The role of the coreceptor Orco in insect olfactory transduction.Journal of comparative physiology. A, Neuroethology, sensory, neural, and behavioral physiology · 2013Review
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Authors and funding
8 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The mechanisms of insect odor transduction are still controversial. Insect odorant receptors (ORs) are 7TM receptors with inverted membrane topology. They colocalize with a conserved coreceptor (Orco) with chaperone and ion channel function. Some studies suggest that insects employ exclusively ionotropic odor transduction via OR-Orco heteromers. Other studies provide evidence for different metabotropic odor transduction cascades, which employ second messenger-gated ion channel families for odor transduction. The hawkmoth Manduca sexta is an established model organism for studies of insect olfaction, also due to the availability of the hawkmoth-specific pheromone blend with its main component bombykal. Previous patch-clamp studies on primary cell cultures of M. sexta olfactory receptor neurons provided evidence for a pheromone-dependent activation of a phospholipase Cβ. Pheromone application elicited a sequence of one rapid, apparently IP3-dependent, transient and two slower Ca(2+)-dependent inward currents. It remains unknown whether additionally an ionotropic pheromone-transduction mechanism is employed. If indeed an OR-Orco ion channel complex underlies an ionotropic mechanism, then Orco agonist-dependent opening of the OR-Orco channel pore should add up to pheromone-dependent opening of the pore. Here, in tip-recordings from intact pheromone-sensitive sensilla, perfusion with the Orco agonist VUAA1 did not increase pheromone-responses within the first 1000 ms. However, VUAA1 increased spontaneous activity of olfactory receptor neurons Zeitgebertime- and dose-dependently. We conclude that we find no evidence for an Orco-dependent ionotropic pheromone transduction cascade in M. sexta. Instead, in M. sexta Orco appears to be a slower, second messenger-dependent pacemaker channel which affects kinetics and threshold of pheromone-detection via changes of intracellular Ca(2+) baseline concentrations.
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