ArticlePloS one2013
Validation of an excretory/secretory antigen based-ELISA for the diagnosis of Opisthorchis felineus infection in humans from low trematode endemic areas.
Article in PloS one, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed.
- Article
- Clonorchiasis and opisthorchiasis: epidemiology, transmission, clinical features, morbidity, diagnosis, treatment, and control.Clinical microbiology reviews · 2024Review
- Automatic detection ofPeerJ · 2024Article
- Diagnosis of Human Trematode Infections.Advances in experimental medicine and biology · 2024Review
- Novel recombinant proteins and peptides fromBiochemistry and biophysics reports · 2023Article
- An Unexpected Case ofPathogens (Basel, Switzerland) · 2023Article
- Infection with Opisthorchis felineus induces intraepithelial neoplasia of the biliary tract in a rodent model.Carcinogenesis · 2017Article
- Standardization of dot-enzyme-linked immmunosorbent assay for the diagnosis of bovine visceral schistosomiasis.Veterinary world · 2017Article
- Enzyme-linked immunosorbent assay for diagnosis of Amphimerus spp. liver fluke infection in Humans.Memorias do Instituto Oswaldo Cruz · 2017Article
- New Perspectives on the Diagnosis of Allergy to Anisakis spp.Current allergy and asthma reports · 2017Review
- Efficacy of Dot-ELISA using different antigens in detecting anti-schistosome antibodies among bovines in field conditions.Journal of parasitic diseases : official organ of the Indian Society for Parasitology · 2016Article
- Trematode Fluke Procerovum varium as Cause of Ocular Inflammation in Children, South India.Emerging infectious diseases · 2016Article
- Cloning and characterization of a novel sigma-like glutathione S-transferase from the giant panda parasitic nematode, Baylisascaris schroederi.Parasites & vectors · 2015Article
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4 authors.
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Abstract
Since opisthorchiasis does not show pathognomonic signs or symptoms, physicians can have serious problems to make a differential diagnosis of this infection in non endemic areas, in particular when there is a simultaneous occurrence with other seasonal infections. Moreover, symptomatic infections due to O. felineus can last a few weeks and then the signs and symptoms disappear, but the worms survive in the bile ducts for years causing hepatobiliary diseases including hepatomegaly, cholangitis, fibrosis of the periportal system, cholecystitis, and gallstones. Consequently, an early diagnosis prevents chronicity and loss of working days. The detection of specific antibodies has been considered as a complementary tool to the fecal examination to establish the definitive diagnosis of this infection and for the follow up. Therefore the aim of this work was the development and validation of an enzyme-linked immunosorbent assay (ELISA) using excretory/secretory antigens (ESA) from O. felineus adult worms to detect anti-Opisthorchis IgG in human sera. A total of 370 human sera were tested: 144 sera from persons with a confirmed diagnosis of opisthorchiasis, 110 sera from healthy Italian people, and 116 sera from people with other parasitic or non-parasitic infections. Results were analyzed by receiver-operator characteristic (ROC) curve analysis. The accuracy of the test, calculated by the area under curve (AUC), yielded a 0.999 value, indicating the high performance of the test. The sensitivity was 100% (95% CI: 97.40% to 100%) and no false-negative sera were detected; the specificity was 99.09% (95% CI: 95.02% to 99.83%). The validated ELISA shows a good performance in terms of sensitivity, repeatability and reproducibility, and it is suitable to detect anti-Opisthorchis IgG in human sera for diagnostic purposes and for the follow up to assess the efficacy of drug treatment.
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