ArticleProceedings of the National Academy of Sciences of the United States of America2013
Ribonucleolytic resection is required for repair of strand displaced nonhomologous end-joining intermediates.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 31 papers.
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Who cites it
31 citing papers in PubMed, 51 citations in OpenAlex.
- Insights into the functional coordination of LigD and Ku in bacterial nonhomologous end joining.Scientific reports · 2026Article
- Asgard archaea: have we found our microbial ancestors?The EMBO journal · 2026Review
- Archaeal replicative primase mediates DNA double-strand break repair.Nucleic acids research · 2025Article
- Editing microbes to mitigate enteric methane emissions in livestock.World journal of microbiology & biotechnology · 2024Review
- Take a Break to Repair: A Dip in the World of Double-Strand Break Repair Mechanisms Pointing the Gaze on Archaea.International journal of molecular sciences · 2021Review
- The Ku complex: recent advances and emerging roles outside of non-homologous end-joining.Cellular and molecular life sciences : CMLS · 2021Review
- The Multifaceted Roles of Ku70/80.International journal of molecular sciences · 2021Review
- Evolutionary and Comparative Analysis of Bacterial Nonhomologous End Joining Repair.Genome biology and evolution · 2020Article
- Archaeal DNA Repair Mechanisms.Biomolecules · 2020Review
- Article
- Structural Determinants Responsible for the Preferential Insertion of Ribonucleotides by Bacterial NHEJ PolDom.Biomolecules · 2020Article
- Causes and Effects of Loss of Classical Nonhomologous End Joining Pathway in Parasitic Eukaryotes.mBio · 2019Article
- Bacterial Ligase D preternary-precatalytic complex performs efficient abasic sites processing at double strand breaks during nonhomologous end joining.Nucleic acids research · 2019Article
- Physical and functional interplay between PCNA DNA clamp and Mre11-Rad50 complex from the archaeon Pyrococcus furiosus.Nucleic acids research · 2018Article
- DNA Ligase C and Prim-PolC participate in base excision repair in mycobacteria.Nature communications · 2017Article
- Structural accommodation of ribonucleotide incorporation by the DNA repair enzyme polymerase Mu.Nucleic acids research · 2017Article
- Cas9-mediated genome editing in the methanogenic archaeonProceedings of the National Academy of Sciences of the United States of America · 2017Article
- Biochemical and Structural Characterisation of DNA Ligases from Bacteria and Archaea.Bioscience reports · 2016Article
- C-terminal region of bacterial Ku controls DNA bridging, DNA threading and recruitment of DNA ligase D for double strand breaks repair.Nucleic acids research · 2016Article
- Molecular basis for DNA strand displacement by NHEJ repair polymerases.Nucleic acids research · 2016Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
Nonhomologous end-joining (NHEJ) pathways repair DNA double-strand breaks (DSBs) in eukaryotes and many prokaryotes, although it is not reported to operate in the third domain of life, archaea. Here, we describe a complete NHEJ complex, consisting of DNA ligase (Lig), polymerase (Pol), phosphoesterase (PE), and Ku from a mesophillic archaeon, Methanocella paludicola (Mpa). Mpa Lig has limited DNA nick-sealing activity but is efficient in ligating nicks containing a 3' ribonucleotide. Mpa Pol preferentially incorporates nucleoside triphosphates onto a DNA primer strand, filling DNA gaps in annealed breaks. Mpa PE sequentially removes 3' phosphates and ribonucleotides from primer strands, leaving a ligatable terminal 3' monoribonucleotide. These proteins, together with the DNA end-binding protein Ku, form a functional NHEJ break-repair apparatus that is highly homologous to the bacterial complex. Although the major roles of Pol and Lig in break repair have been reported, PE's function in NHEJ has remained obscure. We establish that PE is required for ribonucleolytic resection of RNA intermediates at annealed DSBs. Polymerase-catalyzed strand-displacement synthesis on DNA gaps can result in the formation of nonligatable NHEJ intermediates. The function of PE in NHEJ repair is to detect and remove inappropriately incorporated ribonucleotides or phosphates from 3' ends of annealed DSBs to configure the termini for ligation. Thus, PE prevents the accumulation of abortive genotoxic DNA intermediates arising from strand displacement synthesis that otherwise would be refractory to repair.
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