ArticleProceedings of the National Academy of Sciences of the United States of America2013
Calcium signaling mediates cold sensing in insect tissues.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 34 papers, 1 of them a synthesis that pooled it.
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Who cites it
34 citing papers in PubMed, 1 synthesis or guideline pooled it, 106 citations in OpenAlex.
- Pooled it
- Convergent elevation of mitochondrial proton leakage improves thermogenesis in honeybees.Molecular biology and evolution · 2026Article
- Changes in Transcriptome and Functional Evaluation of Heat Shock Protein 70 in Predatory MiteInsects · 2026Article
- Lipid Metabolism in Relation to Calcium Homeostasis.Advances in experimental medicine and biology · 2026Review
- Heat-off responses of epidermal cells sensitizebioRxiv : the preprint server for biology · 2025Article
- Article
- Genome-wide identification of PAR domain protein 1 (PDP1) targets through ChIP-seq reveals the regulation of diapause-specific characteristics in Culex pipiens.Insect molecular biology · 2024Article
- Transcriptome and Expression Analysis of Glycerol Biosynthesis-Related Genes inInternational journal of molecular sciences · 2024Article
- Diapause-Linked Gene Expression Pattern and Related Candidate Duplicated Genes of the Mountain ButterflyInternational journal of molecular sciences · 2023Article
- Cold-induced [Ca2+]cyt elevations function to support osmoregulation in marine diatoms.Plant physiology · 2022Article
- Transcriptome analysis of the response to low temperature acclimation in Calliptamus italicus eggs.BMC genomics · 2022Article
- A Comparative Perspective on Functionally-Related, Intracellular Calcium Channels: The Insect Ryanodine and Inositol 1,4,5-Trisphosphate Receptors.Biomolecules · 2021Review
- Identification of a neural basis for cold acclimation iniScience · 2021Article
- Generation of GCaMP6s-Expressing Zebrafish to Monitor Spatiotemporal Dynamics of Calcium Signaling Elicited by Heat Stress.International journal of molecular sciences · 2021Article
- NaScience advances · 2021Article
- Characterization of Biological Pathways Regulating Acute Cold Resistance of Zebrafish.International journal of molecular sciences · 2021Article
- Article
- miR-31-5p regulates cold acclimation of the wood-boring beetle Monochamus alternatus via ascaroside signaling.BMC biology · 2020Article
- Monarch butterflies use an environmentally sensitive, internal timer to control overwintering dynamics.Molecular ecology · 2019Article
- Understanding the regulation of overwintering diapause molecular mechanisms in Culex pipiens pallens through comparative proteomics.Scientific reports · 2019Article
Corrections and comments
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Authors and funding
4 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The ability to rapidly respond to changes in temperature is a critical adaptation for insects and other ectotherms living in thermally variable environments. In a process called rapid cold hardening (RCH), insects significantly enhance cold tolerance following brief (i.e., minutes to hours) exposure to nonlethal chilling. Although the ecological relevance of RCH is well-established, the underlying physiological mechanisms that trigger RCH are poorly understood. RCH can be elicited in isolated tissues ex vivo, suggesting cold-sensing and downstream hardening pathways are governed by brain-independent signaling mechanisms. We previously provided preliminary evidence that calcium is involved in RCH, and here we firmly establish that calcium signaling mediates cold sensing in insect tissues. In tracheal cells of the freeze-tolerant goldenrod gall fly, Eurosta solidaginis, chilling to 0 °C evoked a 40% increase in intracellular calcium concentration as determined by live-cell confocal imaging. Downstream of calcium entry, RCH conditions significantly increased the activity of calcium/calmodulin-dependent protein kinase II (CaMKII) while reducing phosphorylation of the inhibitory Thr306 residue. Pharmacological inhibitors of calcium entry, calmodulin activation, and CaMKII activity all prevented ex vivo RCH in midgut and salivary gland tissues, indicating that calcium signaling is required for RCH to occur. Similar results were obtained for a freeze-intolerant species, adults of the flesh fly, Sarcophaga bullata, suggesting that calcium-mediated cold sensing is a general feature of insects. Our results imply that insect tissues use calcium signaling to instantly detect decreases in temperature and trigger downstream cold-hardening mechanisms.
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