ArticlePlant physiology2013
GoldenBraid 2.0: a comprehensive DNA assembly framework for plant synthetic biology.
Article in Plant physiology, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 237 papers.
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237 citing papers in PubMed.
- Bxb1 and ParA recombinase-based gene assembly for multiple genes expression in plant cells.Plant physiology · 2026Article
- Advances in large DNA fragment assembly for microbial cell factory engineering.Quantitative biology (Beijing, China) · 2026Review
- Unexpected Suppression of Tomato Fruit Cuticle Formation by Overexpression of a Key Cutin Biosynthetic Enzyme.Plant biotechnology journal · 2026Article
- GH3 phylogenetic subgroups define divergent routes of auxin inactivation via aspartate and glutamine conjugation.Plant physiology · 2026Article
- Polymorphism can extensively reshape the genome-wide crossover landscape in Arabidopsis thaliana.Nature communications · 2026Article
- Rapid reconstitution and kinase-controlled regulation of algal pyrenoid condensates in engineeredbioRxiv : the preprint server for biology · 2026Article
- The plant Golden Gate toolkit: compatibility, interoperability, and harmonized assembly in plant synthetic biology.Plant methods · 2026Review
- Dynamic assembly of malate dehydrogenase-citrate synthase multienzyme complex in the mitochondria.eLife · 2026Article
- Altering the dosage of meiotic crossover-associated RING finger proteins affects crossover number and interference in Drosophila.G3 (Bethesda, Md.) · 2026Article
- Root developmental zonation is independent of cell wall pH.Nature plants · 2026Article
- Targeted DNA ADP-ribosylation triggers templated repair in bacteria and base mutagenesis in eukaryotes.Nature biotechnology · 2026Article
- The grapevine LysM receptor kinase VvLYK4-2 is a key player in chitosan-triggered immune responses.Horticulture research · 2026Article
- dSaCas9 enables enhanced transcriptional activation in Nicotiana benthamiana compared to its dSpCas9 ortholog.BMC plant biology · 2026Article
- Development of the HTRF assay to evaluate the auxin-induced binding between TIR1 and IAA7.The New phytologist · 2026Article
- Bioluminescent sentinel plants enable autonomous diagnostics of viral infections.Nature communications · 2026Article
- Natural variation links seedling hypocotyl growth with water loss in Arabidopsis.The Plant journal : for cell and molecular biology · 2026Article
- Non-invasive imaging of defence responses in plants.Nature communications · 2026Article
- Combinatorial screening in tobacco enables engineering of rose-terpenoid-enriched rice endosperm.Plant communications · 2026Article
- Transgenic Tobacco as a Bioreactor for the Production of Bioactive and Triple-Helical Recombinant Type III Collagen.Plants (Basel, Switzerland) · 2026Article
- The Coli Toolkit (CTK): An Extension of the Modular Yeast Toolkit for Use inACS synthetic biology · 2026Article
177 more citing papers are in PubMed but not listed here.
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Authors and funding
9 authors.
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Abstract
Plant synthetic biology aims to apply engineering principles to plant genetic design. One strategic requirement of plant synthetic biology is the adoption of common standardized technologies that facilitate the construction of increasingly complex multigene structures at the DNA level while enabling the exchange of genetic building blocks among plant bioengineers. Here, we describe GoldenBraid 2.0 (GB2.0), a comprehensive technological framework that aims to foster the exchange of standard DNA parts for plant synthetic biology. GB2.0 relies on the use of type IIS restriction enzymes for DNA assembly and proposes a modular cloning schema with positional notation that resembles the grammar of natural languages. Apart from providing an optimized cloning strategy that generates fully exchangeable genetic elements for multigene engineering, the GB2.0 toolkit offers an evergrowing open collection of DNA parts, including a group of functionally tested, premade genetic modules to build frequently used modules like constitutive and inducible expression cassettes, endogenous gene silencing and protein-protein interaction tools, etc. Use of the GB2.0 framework is facilitated by a number of Web resources that include a publicly available database, tutorials, and a software package that provides in silico simulations and laboratory protocols for GB2.0 part domestication and multigene engineering. In short, GB2.0 provides a framework to exchange both information and physical DNA elements among bioengineers to help implement plant synthetic biology projects.
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