ArticleMolecular & cellular proteomics : MCP2013
A fast workflow for identification and quantification of proteomes.
Article in Molecular & cellular proteomics : MCP, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 47 papers.
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Who cites it
47 citing papers in PubMed.
- DiNovo enables high-coverage and high-confidence de novo peptide sequencing via mirror proteases and deep learning.Nature communications · 2026Article
- The coming era of proteomics-driven precision medicine.National science review · 2025Review
- Integrated proteomics and scRNA-seq analyses of ovarian cancer reveal molecular subtype-associated cell landscapes and immunotherapy targets.British journal of cancer · 2025Article
- Article
- Quartet protein reference materials and datasets for multi-platform assessment of label-free proteomics.Genome biology · 2023Article
- Multilevel proteomic analyses reveal molecular diversity between diffuse-type and intestinal-type gastric cancer.Nature communications · 2023Article
- Comparative proteomic analysis of children FSGS FFPE tissues.BMC pediatrics · 2022Article
- Comparative proteomics profiling revealed the involvement of GRB2-ROCK2 axis in Lyme neuroborreliosis caused by Borrelia Burgdorferi.Journal of cellular and molecular medicine · 2022Article
- Study on diverse pathological characteristics of heart failure in different stages based on proteomics.Journal of cellular and molecular medicine · 2022Article
- Investigating changes of proteome in the bovine milk serum after retort processing using proteomics techniques.Food science & nutrition · 2022Article
- Profiling of Protein Carbonylations in Ferroptosis by Chemical Proteomics.Methods in molecular biology (Clifton, N.J.) · 2022Article
- Proteomics provides individualized options of precision medicine for patients with gastric cancer.Science China. Life sciences · 2021Article
- A time-resolved multi-omic atlas of the developing mouse liver.Genome research · 2020Article
- Transforming growth factor (TGF)-β1-induced miR-133a inhibits myofibroblast differentiation and pulmonary fibrosis.Cell death & disease · 2019Article
- Identification of Insulin-Responsive Transcription Factors That Regulate Glucose Production by Hepatocytes.Diabetes · 2019Article
- Transketolase (TKT) activity and nuclear localization promote hepatocellular carcinoma in a metabolic and a non-metabolic manner.Journal of experimental & clinical cancer research : CR · 2019Article
- PrecisionMolecular & cellular proteomics : MCP · 2019Article
- Global phosphoproteomic analysis reveals ARMC10 as an AMPK substrate that regulates mitochondrial dynamics.Nature communications · 2019Article
- A region-resolved mucosa proteome of the human stomach.Nature communications · 2019Article
- A time-resolved multi-omic atlas of the developing mouse stomach.Nature communications · 2018Article
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Authors and funding
21 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The current in-depth proteomics makes use of long chromatography gradient to get access to more peptides for protein identification, resulting in covering of as many as 8000 mammalian gene products in 3 days of mass spectrometer running time. Here we report a fast sequencing (Fast-seq) workflow of the use of dual reverse phase high performance liquid chromatography - mass spectrometry (HPLC-MS) with a short gradient to achieve the same proteome coverage in 0.5 day. We adapted this workflow to a quantitative version (Fast quantification, Fast-quan) that was compatible to large-scale protein quantification. We subjected two identical samples to the Fast-quan workflow, which allowed us to systematically evaluate different parameters that impact the sensitivity and accuracy of the workflow. Using the statistics of significant test, we unraveled the existence of substantial falsely quantified differential proteins and estimated correlation of false quantification rate and parameters that are applied in label-free quantification. We optimized the setting of parameters that may substantially minimize the rate of falsely quantified differential proteins, and further applied them on a real biological process. With improved efficiency and throughput, we expect that the Fast-seq/Fast-quan workflow, allowing pair wise comparison of two proteomes in 1 day may make MS available to the masses and impact biomedical research in a positive way.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.