ArticleBiological chemistry2012
Three-dimensional cultures modeling premalignant progression of human breast epithelial cells: role of cysteine cathepsins.
Article in Biological chemistry, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
20 citing papers in PubMed, 30 citations in OpenAlex.
- One-pot method for preparing DNA, RNA, and protein for multiomics analysis.Communications biology · 2024Article
- Association between various cathepsins and uterine leiomyoma: A Mendelian randomization analysis.PloS one · 2024Article
- An AND-gate bioluminescent probe for precise tumor imaging.Chemical science · 2023Article
- Self-assembling peptides with hBMP7 biological activity promote the differentiation of ADSCs into nucleus pulposus-like cells.Journal of orthopaedic surgery and research · 2022Article
- Trehalose Reduces the Secreted Beta-Amyloid Levels in Primary Neurons Independently of Autophagy Induction.Metabolites · 2021Article
- Externally added cystatin C reduces growth of A375 melanoma cells by increasing cell cycle time.FEBS open bio · 2021Article
- Identification of Cell Surface Molecules That Determine the Macrophage Activation Threshold Associated With an Early Stage of Malignant Transformation.Frontiers in immunology · 2021Article
- Catch and Release Photosensitizers: Combining Dual-Action Ruthenium Complexes with Protease Inactivation for Targeting Invasive Cancers.Journal of the American Chemical Society · 2018Article
- Modeling the process of human tumorigenesis.Nature communications · 2017Review
- Pathomimetic avatars reveal divergent roles of microenvironment in invasive transition of ductal carcinoma in situ.Breast cancer research : BCR · 2017Article
- Three-dimensional modelling identifies novel genetic dependencies associated with breast cancer progression in the isogenic MCF10 model.The Journal of pathology · 2016Article
- Photoactivated inhibition of cathepsin K in a 3D tumor model.Biological chemistry · 2016Article
- Review
- Cabozantinib (XL184) Inhibits Growth and Invasion of Preclinical TNBC Models.Clinical cancer research : an official journal of the American Association for Cancer Research · 2016Article
- The importance of selecting the appropriate reference genes for quantitative real time PCR as illustrated using colon cancer cells and tissue.F1000Research · 2016Article
- Photodynamic therapy as an effective therapeutic approach in MAME models of inflammatory breast cancer.Breast cancer research and treatment · 2015Article
- Il-6 signaling between ductal carcinoma in situ cells and carcinoma-associated fibroblasts mediates tumor cell growth and migration.BMC cancer · 2015Article
- Cathepsin B-deficient mice as source of monoclonal anti-cathepsin B antibodies.Biological chemistry · 2015Article
- Metabotropic glutamate receptor-1 contributes to progression in triple negative breast cancer.PloS one · 2014Article
- Next-generation sequencing: a powerful tool for the discovery of molecular markers in breast ductal carcinoma in situ.Expert review of molecular diagnostics · 2013Review
Corrections and comments
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Authors and funding
6 authors at 2 institutions in 1 country.
Funding
Abstract
The expression of the cysteine protease cathepsin B is increased in early stages of human breast cancer.To assess the potential role of cathepsin B in premalignant progression of breast epithelial cells, we employed a 3D reconstituted basement membrane overlay culture model of MCF10A human breast epithelial cells and isogenic variants that replicate the in vivo phenotypes of hyper plasia(MCF10AneoT) and atypical hyperplasia (MCF10AT1). MCF10A cells developed into polarized acinar structures with central lumens. In contrast, MCF10AneoT and MCF10AT1 cells form larger structures in which the lumens are filled with cells. CA074Me, a cell-permeable inhibitor selective for the cysteine cathepsins B and L,reduced proliferation and increased apoptosis of MCF10A, MCF10AneoT and MCF10AT1 cells in 3D culture. We detected active cysteine cathepsins in the isogenic MCF10 variants in 3D culture with GB111, a cell-permeable activity based probe, and established differential inhibition of cathepsin B in our 3D cultures. We conclude that cathepsin B promotes proliferation and premalignant progression of breast epithelial cells. These findings are consistent with studies by others showing that deletion of cathepsin B in the transgenic MMTV-PyMT mice, a murine model that is predisposed to development of mammary cancer, reduces malignant progression.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.