Evidence map›Paper›PMID 23667548›Full record

ArticlePloS one2013

Highly efficient NMR assignment of intrinsically disordered proteins: application to B- and T cell receptor domains.

Linnéa Isaksson, Maxim Mayzel, Maria Saline, Anders Pedersen, Joakim Rosenlöw, Bernhard Brutscher, B Göran Karlsson, Vladislav Y Orekhov

Abstract read
In one paragraph

Article in PloS one, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 30 papers.

0numbers the graph read from it
0cells of the map it votes in
30citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

30 citing papers in PubMed.

  1. Article
  2. Article
  3. Article
  4. Article
  5. Biomolecular NMR assignments · 2022
    Article
  6. Unambiguous Tracking of Protein Phosphorylation by Fast High-Resolution FOSY NMR*.Angewandte Chemie (International ed. in English) · 2021
    Article
  7. Article
  8. Article
  9. Article
  10. Article
  11. Article
  12. Article
  13. Article
  14. Article
  15. Article
  16. Article
  17. Measurement of protein backboneJournal of biomolecular NMR · 2017
    Article
  18. Article
  19. Article
  20. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors.

Linnéa IsakssonSwedish NMR Centre, University of Gothenburg, Gothenburg, Sweden.
Maxim Mayzel
Maria Saline
Anders Pedersen
Joakim Rosenlöw
Bernhard Brutscher
B Göran Karlsson
Vladislav Y Orekhov

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

We present an integrated approach for efficient characterization of intrinsically disordered proteins. Batch cell-free expression, fast data acquisition, automated analysis, and statistical validation with data resampling have been combined for achieving cost-effective protein expression, and rapid automated backbone assignment. The new methodology is applied for characterization of five cytosolic domains from T- and B-cell receptors in solution.

Indexed as

Amino Acid MotifsCytosolHumansIntracellular SpaceIntrinsically Disordered ProteinsLigandsNuclear Magnetic Resonance, BiomolecularProtein Structure, TertiaryReceptors, Antigen, B-CellReceptors, Antigen, T-CellSignal TransductionIntrinsically Disordered ProteinsLigandsReceptors, Antigen, B-CellReceptors, Antigen, T-Cell

Identifiers

PMID23667548
PMCPMC3647075

What OpenQuestion holds

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LicenceCC BY
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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.