ArticlePloS one2013
Loss of the Arabidopsis thaliana P₄-ATPase ALA3 reduces adaptability to temperature stresses and impairs vegetative, pollen, and ovule development.
Article in PloS one, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
29 citing papers in PubMed, 43 citations in OpenAlex.
- Targeted population genomics uncovers demographic history and genetic divergence in north American wild cranberry.Horticulture research · 2026Article
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- Genome-wide identification of the P4ATPase gene family and its response to biotic and abiotic stress in soybean (Glycine max L.).BMC genomics · 2025Article
- Uptake and Metabolic Conversion of Exogenous Phosphatidylcholines Depending on Their Acyl Chain Structure inInternational journal of molecular sciences · 2023Article
- Diversity and genetic architecture of agro-morphological traits in a core collection of European traditional tomato.Journal of experimental botany · 2023Article
- Arabidopsis flippase ALA3 is required for adjustment of early subcellular trafficking in plant response to osmotic stress.Journal of experimental botany · 2023Article
- Mini-review: Lipid flippases as putative targets for biotechnological crop improvement.Frontiers in plant science · 2023Review
- A genome-wide association study identifies novel players in Na and Fe homeostasis in Arabidopsis thaliana under alkaline-salinity stress.The Plant journal : for cell and molecular biology · 2023Article
- Genomic-environmental associations in wild cranberry (Vaccinium macrocarpon Ait.).G3 (Bethesda, Md.) · 2022Article
- The phospholipid flippase ALA3 regulates pollen tube growth and guidance in Arabidopsis.The Plant cell · 2022Article
- Comprehensive analysis of glycerolipid dynamics during tobacco pollen germination and pollen tube growth.Frontiers in plant science · 2022Article
- Arabidopsis P4 ATPase-mediated cell detoxification confers resistance to Fusarium graminearum and Verticillium dahliae.Nature communications · 2021Article
- Arabidopsis P4 ATPase-mediated cell detoxification confers resistance to Fusarium graminearum and Verticillium dahliae.Nature communications · 2021Article
- Two Is Company, but Four Is a Party-Challenges of Tetraploidization for Cell Wall Dynamics and Efficient Tip-Growth in Pollen.Plants (Basel, Switzerland) · 2021Review
- Lipid flippases as key players in plant adaptation to their environment.Nature plants · 2021Review
- Dynamic membranes: the multiple roles of P4 and P5 ATPases.Plant physiology · 2021Article
- A potential pathway for flippase-facilitated glucosylceramide catabolism in plants.Plant signaling & behavior · 2020Article
- The Tip-Localized Phosphatidylserine Established by Arabidopsis ALA3 Is Crucial for Rab GTPase-Mediated Vesicle Trafficking and Pollen Tube Growth.The Plant cell · 2020Article
Corrections and comments
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Authors and funding
5 authors at 3 institutions in 2 countries.
Funding
Abstract
Members of the P4 subfamily of P-type ATPases are thought to help create asymmetry in lipid bilayers by flipping specific lipids between the leaflets of a membrane. This asymmetry is believed to be central to the formation of vesicles in the secretory and endocytic pathways. In Arabidopsis thaliana, a P4-ATPase associated with the trans-Golgi network (ALA3) was previously reported to be important for vegetative growth and reproductive success. Here we show that multiple phenotypes for ala3 knockouts are sensitive to growth conditions. For example, ala3 rosette size was observed to be dependent upon both temperature and soil, and varied between 40% and 80% that of wild-type under different conditions. We also demonstrate that ala3 mutants have reduced fecundity resulting from a combination of decreased ovule production and pollen tube growth defects. In-vitro pollen tube growth assays showed that ala3 pollen germinated ∼2 h slower than wild-type and had approximately 2-fold reductions in both maximal growth rate and overall length. In genetic crosses under conditions of hot days and cold nights, pollen fitness was reduced by at least 90-fold; from ∼18% transmission efficiency (unstressed) to less than 0.2% (stressed). Together, these results support a model in which ALA3 functions to modify endomembranes in multiple cell types, enabling structural changes, or signaling functions that are critical in plants for normal development and adaptation to varied growth environments.
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Registered trials
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