ArticlePloS one2013
In vivo intracellular oxygen dynamics in murine brain glioma and immunotherapeutic response of cytotoxic T cells observed by fluorine-19 magnetic resonance imaging.
Article in PloS one, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed.
- Bridging tumor biology and radiomics through an ultra-large physics-driven vascular model.npj biomedical innovations · 2026Article
- Heterogeneity, Measurement, and Clinical Implications of Oxygenation, Cell Signaling, and Redox Biology in Glioblastoma and Adult Diffuse Gliomas, with Context from Other Brain Tumors.Antioxidants (Basel, Switzerland) · 2026Review
- Identifying new therapeutics for focused ultrasound-enhanced drug delivery in the management of glioblastoma.Frontiers in oncology · 2025Review
- Oxygen-Carrying Capacity of Perfluorohexyloctane, a Novel Eye Drop for Dry Eye Disease.Current therapeutic research, clinical and experimental · 2023Article
- Prognostic Value of Fluorine-19 MRI Oximetry Monitoring in cancer.Molecular imaging and biology · 2022Review
- Assessing Oximetry Response to Chimeric Antigen Receptor T-cell Therapy against Glioma withRadiology. Imaging cancer · 2021Article
- A systematic optimization ofNeuroImage · 2019Article
- QuantitativeMagma (New York, N.Y.) · 2019Article
- A dualMagma (New York, N.Y.) · 2019Article
- Fluorine-19 MRI for detection and quantification of immune cell therapy for cancer.Journal for immunotherapy of cancer · 2018Review
- Siloxane Nanoprobes for Labeling and Dual Modality Functional Imaging of Neural Stem Cells.Annals of biomedical engineering · 2016Article
- Advances in using MRI probes and sensors for in vivo cell tracking as applied to regenerative medicine.Disease models & mechanisms · 2015Review
- Fluorine-19 MRI Contrast Agents for Cell Tracking and Lung Imaging.Magnetic resonance insights · 2015Review
- Tracking immune cells in vivo using magnetic resonance imaging.Nature reviews. Immunology · 2013Review
Corrections and comments
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Authors and funding
4 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Noninvasive biomarkers of anti-tumoral efficacy are of great importance to the development of therapeutic agents. Tumor oxygenation has been shown to be an important indicator of therapeutic response. We report the use of intracellular labeling of tumor cells with perfluorocarbon (PFC) molecules, combined with quantitative ¹⁹F spin-lattice relaxation rate (R₁) measurements, to assay tumor cell oxygen dynamics in situ. In a murine central nervous system (CNS) GL261 glioma model, we visualized the impact of Pmel-1 cytotoxic T cell immunotherapy, delivered intravenously, on intracellular tumor oxygen levels. GL261 glioma cells were labeled ex vivo with PFC and inoculated into the mouse striatum. The R₁ of ¹⁹F labeled cells was measured using localized single-voxel magnetic resonance spectroscopy, and the absolute intracellular partial pressure of oxygen (pO₂) was ascertained. Three days after tumor implantation, mice were treated with 2×10⁷ cytotoxic T cells intravenously. At day five, a transient spike in pO₂ was observed indicating an influx of T cells into the CNS and putative tumor cell apoptosis. Immunohistochemistry and quantitative flow cytometry analysis confirmed that the pO₂ was causally related to the T cells infiltration. Surprisingly, the pO₂ spike was detected even though few (∼4×10⁴) T cells actually ingress into the CNS and with minimal tumor shrinkage. These results indicate the high sensitivity of this approach and its utility as a non-invasive surrogate biomarker of anti-cancer immunotherapeutic response in preclinical models.
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